Anti-Desmin Antibody Picoband® (monoclonal, 2B5)

About this product

Boster Bio Anti-Desmin Antibody Picoband® (monoclonal, 2B5) catalog # M01948-3. Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

M01948-3

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Synonyms

Desmin; DES

Gene Name

DES

Gene ID

1674

UniProt

P17661

Host

Mouse

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human Desmin recombinant protein (Position: M1-T304) . Human Desmin shares 97% amino acid (aa) sequence identity with both mouse and rat Desmin.

Clonality

Monoclonal

Clone

Clone: 2B5

Applications

Flow Cytometry, IHC, WB

Field of Research

Cancer, Cell Biology, Cell Cycle, Cell Division,2339, Chromosome Structure, DNA/RNA, DNA Damage & Repair, DNA Damage Response, Epigenetics and Nuclear Signaling

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500μg/ml.

Function

Muscle-specific type III intermediate filament essential for proper muscular structure and function. Plays a crucial role in maintaining the structure of sarcomeres, inter-connecting the Z-disks and forming the myofibrils, linking them not only to the sarcolemmal cytoskeleton, but also to the nucleus and mitochondria, thus providing strength for the muscle fiber during activity. In adult striated muscle they form a fibrous network connecting myofibrils to each other and to the plasma membrane from the periphery of the Z-line structures. May act as a sarcomeric microtubule-anchoring protein: specifically associates with detyrosinated tubulin-alpha chains, leading to buckled microtubules and mechanical resistance to contraction. Contributes to the transcriptional regulation of the NKX2-5 gene in cardiac progenitor cells during a short period of cardiomyogenesis and in cardiac side population stem cells in the adult. Plays a role in maintaining an optimal conformation of nebulette (NEB) on heart muscle sarcomeres to bind and recruit cardiac alpha-actin.

References & Citations

1. Muñoz-Mármol AM, Strasser G, Isamat M, Coulombe PA, Yang Y, Roca X, Vela E, Mate JL, Coll J, Fernández-Figueras MT, Navas-Palacios JJ, Ariza A, Fuchs E (September 1998) .A dysfunctional desmin mutation in a patient with severe generalized myopathy. Proc. Natl. Acad. Sci. U.S.A. 95 (19) : 11312–7. 2. Li ZL, Lilienbaum A, Butler-Browne G, Paulin D (May 1989) . Human desmin-coding gene: complete nucleotide sequence, characterization and regulation of expression during myogenesis and development. Gene 78 (2) : 243–54. 3. Tidball, J. G. Desmin at myotendinous junctions. Exp. Cell Res. 199: 206-212, 1992.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Observed Molecular Weight

54 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

Desmin

Subcellular Location

Nucleus. Sarcolemma. Z line. Cytoplasm.

Isotype

Mouse IgG2b

Contents

243