Anti-CD43/SPN Antibody Picoband® (monoclonal, 4I3)

About this product

Boster Bio Anti-CD43/SPN Antibody Picoband® (monoclonal, 4I3) catalog # M01296-1. Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

M01296-1

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

CD43, also known as leukosialin or sialophorin, is a transmembrane cell surface protein that in humans is encoded by the SPN gene. It is mapped to 16p11.2. It is a major sialoglycoprotein on the surface of human T lymphocytes, monocytes, granulocytes, and some B lymphocytes, which is important for immune function and may be part of a physiologic ligand-receptor complex involved in T-cell activation. Expression of CD43 is deficient and/or defective in the X-chromosome-linked immunodeficiency disorder Wiscott-Aldrich syndrome, suggesting that CD43 have a role in T-cell activation. T-cell activation requires the removal of CD43 from the immunologic synapse to allow efficient engagement of the TCR with molecules on the APC.

Synonyms

Leukosialin; GPL115; Galactoglycoprotein; GALGP; Leukocyte sialoglycoprotein; Sialophorin; CD43; CD43 cytoplasmic tail; CD43-ct; CD43ct; SPN; CD43

Gene Name

SPN

Gene ID

6693

UniProt

P16150

Host

Mouse

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human CD43 recombinant protein (Position: A272-P400) . Human CD43 shares 72% and 73% amino acid (aa) sequence identity with mouse and rat CD43, respectively.

Clonality

Monoclonal

Clone

Clone: 4I3

Tissue Specificity

Cell surface of thymocytes, T-lymphocytes, neutrophils, plasma cells and myelomas.

Applications

Flow Cytometry, IHC, WB

Field of Research

Cancer, Cell Biology, Cell Cycle, Cell Cycle Inhibitors, Deubiquitination, Epigenetics and Nuclear Signaling, Host-Virus Interaction, Interspecies Interaction, Microbiology, p53 Pathway, Proteasome / Ubiquitin, Proteolysis/Ubiquitin, Ubiquitin & Ubiquitin Like Modifiers

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500μg/ml.

Function

Predominant cell surface sialoprotein of leukocytes which regulates multiple T-cell functions, including T-cell activation, proliferation, differentiation, trafficking and migration. Positively regulates T-cell trafficking to lymph-nodes via its association with ERM proteins (EZR, RDX and MSN) . Negatively regulates Th2 cell differentiation and predisposes the differentiation of T-cells towards a Th1 lineage commitment. Promotes the expression of IFN-gamma by T-cells during T-cell receptor (TCR) activation of naive cells and induces the expression of IFN-gamma by CD4+ T-cells and to a lesser extent by CD8+ T-cells. Plays a role in preparing T-cells for cytokine sensing and differentiation into effector cells by inducing the expression of cytokine receptors IFNGR and IL4R, promoting IFNGR and IL4R signaling and by mediating the clustering of IFNGR with TCR. Acts as a major E-selectin ligand responsible for Th17 cell rolling on activated vasculature and recruitment during inflammation. Mediates Th17 cells, but not Th1 cells, adhesion to E-selectin. Acts as a T-cell counter-receptor for SIGLEC1. CD43 cytoplasmic tail: Protects cells from apoptotic signals, promoting cell survival.

References & Citations

1. Fukuda M, Carlsson SR (1987) . Leukosialin, a major sialoglycoprotein on human leukocytes as differentiation antigens.. Med. Biol. 64 (6) : 335–43. 2. Rogaev EI, Keryanov SA (1993) . Unusual variability of the complex dinucleotide repeat block at the SPN locus.. Hum. Mol. Genet. 1 (8) : 657. 3. Rosenstein Y, Park JK, Hahn WC, et al. (1992) . CD43, a molecule defective in Wiskott-Aldrich syndrome, binds ICAM-1.. Nature 354 (6350) : 233–5.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Observed Molecular Weight

115 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

Sialophorin

Subcellular Location

Membrane. Single-pass type I membrane protein. Microvillus. Uropodium. Nucleus. PML body.

Isotype

Mouse IgG2b

Contents

243