Anti-COMT Antibody Picoband® (monoclonal, 15C10)

About this product

Boster Bio Anti-COMT Antibody Picoband® (monoclonal, 15C10) catalog # M00464. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

M00464

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Catechol O-methyltransferase, also called COMT, is one of the major mammalian enzymes involved in the metabolic degradation of catecholamines. This gene is mapped to 22q11.21. Catechol-O-methyltransferase catalyzes the transfer of a methyl group from S-adenosylmethionine to catecholamines, including the neurotransmitters dopamine, epinephrine, and norepinephrine. This O-methylation results in one of the major degradative pathways of the catecholamine transmitters. In addition to its role in the metabolism of endogenous substances, COMT is important in the metabolism of catechol drugs used in the treatment of hypertension, asthma, and Parkinson disease. COMT is found in two forms in tissues, a soluble form (S-COMT) and a membrane-bound form (MB-COMT) . The differences between S-COMT and MB-COMT reside within the N-termini.

Synonyms

Catechol O-methyltransferase; COMT

Gene Name

COMT

Gene ID

1312

UniProt

P21964

Host

Mouse

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human COMT recombinant protein (Position: G52-P271) . Human COMT shares 81.9% and 81% amino acid (aa) sequence identity with mouse and rat COMT, respectively.

Clonality

Monoclonal

Clone

Clone: 15C10

Tissue Specificity

Brain, liver, placenta, lymphocytes and erythrocytes.

Applications

Flow Cytometry, IF, IHC, ICC, WB

Field of Research

Cell Adhesion, Cell Type Markers, Cytoskeleton/ECM, Hematopoietic Progenitors, Immunology, Integrins, Myeloid, Signal Transduction, Stem Cells

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500μg/ml.

Function

Catalyzes the O-methylation, and thereby the inactivation, of catecholamine neurotransmitters and catechol hormones. Also shortens the biological half-lives of certain neuroactive drugs, like L-DOPA, alpha-methyl DOPA and isoproterenol.

References & Citations

1. Baker, K., Baldeweg, T., Sivagnanasundaram, S., Scambler, P., Skuse, D. COMT val108/158met modifies mismatch negativity and cognitive function in 22q11 deletion syndrome. Biol. Psychiat. 58: 23-31, 2005. 2. Lee, S.-G., Joo, Y., Kim, B., Chung, S., Kim, H.-L., Lee, I., Choi, B., Kim, C., Song, K.Association of Ala72Ser polymorphism with COMT enzyme activity and the risk of schizophrenia in Koreans. Hum. Genet. 116: 319-328, 2005.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Observed Molecular Weight

29 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

Catechol-O-methyltransferase

Subcellular Location

Cytoplasm. Cell membrane. Single-pass type II membrane protein. Extracellular side.

Protein Name

Integrin beta-2

Isotype

Mouse IgG2b

Contents

243