Anti-ABCB5 Antibody Picoband®

About this product

Boster Bio Anti-ABCB5 Antibody Picoband® catalog # A02979-1. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A02979-1

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

ATP-binding cassette sub-family B member 5 also known as P-glycoprotein ABCB5 is a plasma membrane-spanning protein that in humans is encoded by the ABCB5 gene.It is mapped to 7p21.1. ABCB5 belongs to the ATP-binding cassette (ABC) transporter superfamily of integral membrane proteins. These proteins participate in ATP-dependent transmembrane transport of structurally diverse molecules ranging from small ions, sugars, and peptides to more complex organic molecules.

Synonyms

ATP-binding cassette sub-family B member 5; ABCB5 P-gp; P-glycoprotein ABCB5; ABCB5

Gene Name

ABCB5

Gene ID

340273

UniProt

Q2M3G0

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human ABCB5 recombinant protein (Position: M1-D176) .

Clonality

Polyclonal

Tissue Specificity

Expressed by CD133-expressing progenitor cells among epidermal melanocytes (at protein level) . Widely expressed with specific expression in pigment cells. Highly expressed in several malignant tissues: highly expressed in clinical melanomas, with low expression in normal skin. In melanoma, marks malignant melanoma-initiating cells (MMIC), in which clinical virulence resides as a consequence of unlimited self-renewal capacity, resulting in inexorable tumor progression and metastasis. Also highly expressed in a number of leukemia cells. Expressed in basal limbal epithelium.

Applications

ELISA, Flow Cytometry, IF, IHC, ICC, WB

Field of Research

Cancer, Drug Metabolism, Energy Metabolism, Energy Transfer Pathways, Metabolic Signaling Pathways, Metabolism, Pathways and Processes, Signal Transduction, Tumor Biomarkers

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Drug efflux transporter present in a number of stem cells that acts as a regulator of cellular differentiation. Able to mediate efflux from cells of the rhodamine dye and of the therapeutic drug doxorubicin. Specifically present in limbal stem cells, where it plays a key role in corneal development and repair.

References & Citations

1. Chen, K. G., Szakacs, G., Annereau, J.-P., Rouzaud, F., Liang, X.-J., Valencia, J. C., Nagineni, C. N., Hooks, J. J., Hearing, V. J., Gottesman, M. M. Principal expression of two mRNA isoforms (ABCB5-alpha and ABCB5-beta) of the ATP-binding cassette transporter gene ABCB5 in melanoma cells and melanocytes. Pigment Cell Res. 18: 102-112, 2005. 2. Frank, N. Y., Margaryan, A., Huang, Y., Schatton, T., Waaga-Gasser, A. M., Gasser, M., Sayegh, M. H., Sadee, W., Frank, M. H. ABCB5-mediated doxorubicin transport and chemoresistance in human malignant melanoma. Cancer Res. 65: 4320-4333, 2005.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

36020 MW

Observed Molecular Weight

110 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

ATP binding cassette subfamily B member 5

Subcellular Location

Cell membrane. Multi-pass membrane protein.

Protein Name

Aldo-keto reductase family 1 member B10

Isotype

Rabbit IgG

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.