Anti-DCC Antibody Picoband®

About this product

Boster Bio Anti-DCC Antibody Picoband® catalog # A00560. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A00560

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Deleted in Colorectal Carcinoma, also known as DCC, is a protein which in humans is encoded by the DCC gene. It is mapped to 18q21.2. This gene encodes a netrin 1 receptor. The transmembrane protein is a member of the immunoglobulin superfamily of cell adhesion molecules, and mediates axon guidance of neuronal growth cones towards sources of netrin 1 ligand. The cytoplasmic tail interacts with the tyrosine kinases Src and focal adhesion kinase (FAK, also known as PTK2) to mediate axon attraction. The protein partially localizes to lipid rafts, and induces apoptosis in the absence of ligand. The protein functions as a tumor suppressor, and is frequently mutated or downregulated in colorectal cancer and esophageal carcinoma.

Synonyms

Netrin receptor DCC; Colorectal cancer suppressor; Immunoglobulin superfamily DCC subclass member 1; Tumor suppressor protein DCC; DCC; IGDCC1

Gene Name

DCC

Gene ID

1630

UniProt

P43146

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human DCC recombinant protein (Position: Q851-F1447) .

Clonality

Polyclonal

Tissue Specificity

Found in axons of the central and peripheral nervous system and in differentiated cell types of the intestine. Not expressed in colorectal tumor cells that lost their capacity to differentiate into mucus producing cells.

Applications

ELISA, IHC, WB

Field of Research

Cancer, Cancer Metabolism, Carbohydrate Metabolism, Energy Metabolism, Energy Transfer Pathways, Metabolic Signaling Pathway, Metabolic Signaling Pathways, Metabolism, Metabolism Of Carbohydrates, Pathways and Processes, Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Receptor for netrin required for axon guidance. Mediates axon attraction of neuronal growth cones in the developing nervous system upon ligand binding. Its association with UNC5 proteins may trigger signaling for axon repulsion. It also acts as a dependence receptor required for apoptosis induction when not associated with netrin ligand. Implicated as a tumor suppressor gene.

References & Citations

1. Chan, S. S.-Y., Zheng, H., Su, M.-W., Wilk, R., Killeen, M. T., Hedgecock, E. M., Culotti, J. G. UNC-40, a C. elegans homolog of the DCC (deleted in colorectal cancer), is required in motile cells responding to UNC-6 netrin cues. Cell 87: 187-195, 1996. 2. Cho, K. R., Oliner, J. D., Simons, J. W., Hedrick, L., Fearon, E. R., Preisinger, A. C., Hedge, P., Silverman, G. A., Vogelstein, B. The DCC gene: structural analysis and mutations in colorectal carcinomas. Genomics 19: 525-531, 1994.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

9072 MW

Observed Molecular Weight

180 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

DCC netrin 1 receptor

Subcellular Location

Membrane. Single-pass type I membrane protein.

Protein Name

NEDD8

Isotype

Rabbit IgG

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.