Anti-EIF4A1/2/3 Antibody Picoband®

About this product

Boster Bio Anti-EIF4A1/2/3 Antibody Picoband® catalog # A03922-2. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A03922-2

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Eukaryotic initiation factor 4A-I is a protein that in humans is encoded by the EIF4A1 gene. It is mapped to 17p13.1. EIF4A1 has been shown to interact with EIF4E and eukaryotic translation initiation factor 4 gamma.

Synonyms

Eukaryotic initiation factor 4A-I; eIF-4A-I; eIF4A-I; ATP-dependent RNA helicase eIF4A-1; EIF4A1; DDX2A, EIF4A

Gene Name

EIF4A1

Gene ID

1973

UniProt

P60842

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human EIF4A1/2/3 recombinant protein (Position: D32-I406) .

Clonality

Polyclonal

Tissue Specificity

Adipocytes.

Applications

ELISA, Flow Cytometry, IF, IHC, ICC, WB

Field of Research

Atherosclerosis, Cancer, Cancer Metabolism, Cardiovascular, Heart Disease, Lipid and Lipoprotein Metabolism, Lipid Metabolism, Lipids/Lipoproteins, Metabolic Signaling Pathway, Metabolic Signaling Pathways, Metabolism, Metabolism of Lipids and Lipoproteins, Pathways and Processes, Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon.

References & Citations

1. Garcia-Garcia, C., Frieda, K. L., Feoktistova, K., Fraser, C. S., Block, S. M. Factor-dependent processivity in human eIF4A DEAD-box helicase. Science 348: 1486-1488, 2015. 2. Jones, E., Quinn, C. M., See, C. G., Montgomery, D. S., Ford, M. J., Kolble, K., Gordon, S., Greaves, D. R. The linked human elongation initiation factor 4A1 (EIF4A1) and CD68 genes map to chromosome 17p13. Genomics 53: 248-250, 1998. 3. Kim, N.-S., Kato, T., Abe, N., Kato, S. Nucleotide sequence of human cDNA encoding eukaryotic initiation factor 4A1. Nucleic Acids Res. 21: 2012 only, 1993.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Observed Molecular Weight

46 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

Eukaryotic translation initiation factor 4A1

Subcellular Location

Cytosol. Extracellular exosome. Cytoplasm. Eukaryotic translation initiation factor 4F complex. Membrane.

Isotype

Rabbit IgG

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.