Anti-EIF4G1 Antibody Picoband®

About this product

Boster Bio Anti-EIF4G1 Antibody Picoband® catalog # A01351-2. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A01351-2

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Eukaryotic translation initiation factor 4 gamma 1 is a protein that in humans is encoded by the EIF4G1 gene. It is mapped to 3q27.1. The protein encoded by this gene is a component of the multi-subunit protein complex EIF4F. This complex facilitates the recruitment of mRNA to the ribosome, which is a rate-limiting step during the initiation phase of protein synthesis. The recognition of the mRNA cap and the ATP-dependent unwinding of 5'-terminal secondary structure is catalyzed by factors in this complex. The subunit encoded by this gene is a large scaffolding protein that contains binding sites for other members of the EIF4F complex. A domain at its N-terminus can also interact with the poly (A) -binding protein, which may mediate the circularization of mRNA during translation. Alternative splicing results in multiple transcript variants, some of which are derived from alternative promoter usage.

Synonyms

Eukaryotic translation initiation factor 4 gamma 1; eIF-4-gamma 1; eIF-4G 1; eIF-4G1; p220; EIF4G1; EIF4F; EIF4G; EIF4GI

Gene Name

EIF4G1

Gene ID

1981

UniProt

Q04637

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

Human EIF4G1

Clonality

Polyclonal

Tissue Specificity

Expressed abundantly in the bone marrow. Expressed exclusively by eosinophils and basophils. Not detected in monocytes and neutrophils. Expressed in CD25-positive regulatory T-cells (Treg) (at protein level) . Found in intestinal tissue from patients with Celiac disease, expression is ly related to the histological grade of mucosal damage and to the number of eosinophils found in the duodenal lesion (at protein level) . Found in sputum of patients with eosinophilic inflammatory diseases such as asthma (at protein level) .

Applications

ELISA, Flow Cytometry, IF, IHC, ICC, WB

Field of Research

Cancer, Channels, Cytoskeleton/ECM, ECM Proteins, Extracellular Matrix, Metabolism, Plasma Membrane, Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Component of the protein complex eIF4F, which is involved in the recognition of the mRNA cap, ATP-dependent unwinding of 5'-terminal secondary structure and recruitment of mRNA to the ribosome.

References & Citations

1. Huttenlocher, J., Kruger, R., Capetian, P., Lohmann, K., Brockmann, K., Csoti, I., Klein, C., Berg, D., Gasser, T., Bonin, M., Riess, O., Bauer, P. EIF4G1 is neither a strong nor a common risk factor for Parkinson's disease: evidence from large European cohorts. J. Med. Genet. 52: 37-41, 2015. 2. Imataka, H., Gradi, A., Sonenberg, N. A newly identified N-terminal amino acid sequence of human eIF4G binds poly (A) -binding protein and functions in poly (A) -dependent translation. EMBO J. 17: 7480-7489, 1998.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Observed Molecular Weight

250 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

Western blot, 0.25-0.5μg/mL, Human, Mouse, Rat<br> Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/mL, Human, Mouse, Rat<br> Immunocytochemistry/Immunofluorescence, 2μg/mL, Human<br> Flow Cytometry (Fixed), 1-3μg/1x10⁶ cells, Human<br> ELISA, 0.1-0.5μg/mL, -<br>

Gene Name Synonym

Eukaryotic translation initiation factor 4 gamma 1

Subcellular Location

Cytosol. Nucleus. Cytoplasm. Eukaryotic translation initiation factor 4F complex. Membrane. Polysome.

Isotype

Rabbit IgG

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.