Anti-B7H4/VTCN1 Antibody Picoband®

About this product

Boster Bio Anti-B7H4/VTCN1 Antibody Picoband® catalog # A02821-3. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A02821-3

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

V-set domain-containing T-cell activation inhibitor 1 is a protein that in humans is encoded by the VTCN1 gene. It is mapped to 1p13.1-p12. This gene encodes a protein belonging to the B7 costimulatory protein family. Proteins in this family are present on the surface of antigen-presenting cells and interact with ligand bound to receptors on the surface of T cells. Studies have shown that high levels of the encoded protein has been correlated with tumor progression. A pseudogene of this gene is located on chromosome 20. Multiple transcript variants encoding different isoforms have been found for this gene.

Synonyms

V-set domain-containing T-cell activation inhibitor 1; B7 homolog 4; B7-H4; B7h.5; Immune costimulatory protein B7-H4; Protein B7S1; T-cell costimulatory molecule B7x; VTCN1; B7H4; UNQ659; PRO1291

Gene Name

VTCN1

Gene ID

79679

UniProt

Q7Z7D3

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human B7H4/VTCN1 recombinant protein (Position: T57-A258) .

Clonality

Polyclonal

Tissue Specificity

Overexpressed in breast, ovarian, endometrial, renal cell (RCC) and non-small-cell lung cancers (NSCLC) . Expressed on activated T- and B-cells, monocytes and dendritic cells, but not expressed in most normal tissues (at protein level) . Widely expressed, including in kidney, liver, lung, ovary, placenta, spleen and testis.

Applications

ELISA, Flow Cytometry, IF, IHC, ICC, WB

Field of Research

Cell Biology, Co-Activators/Co-Repressors, Epigenetics and Nuclear Signaling, Nuclear Receptors, Nuclear Signaling Pathways, Proteasome / Ubiquitin, Proteolysis/Ubiquitin, Transcription, Transcription Factors, Ubiquitin & Ubiquitin Like Modifiers

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Negatively regulates T-cell-mediated immune response by inhibiting T-cell activation, proliferation, cytokine production and development of cytotoxicity. When expressed on the cell surface of tumor macrophages, plays an important role, together with regulatory T-cells (Treg), in the suppression of tumor-associated antigen-specific T-cell immunity. Involved in promoting epithelial cell transformation.

References & Citations

1. Krambeck, A. E., Thompson, R. H., Dong, H., Lohse, C. M., Park, E. S., Kuntz, S. M., Leibovich, B. C., Blute, M. L., Cheville, J. C., Kwon, E. D. B7-H4 expression in renal cell carcinoma and tumor vasculature: associations with cancer progression and survival. Proc. Nat. Acad. Sci. 103: 10391-10396, 2006. 2. Kryczek, I., Zou, L., Rodriguez, P., Zhu, G., Wei, S., Mottram, P., Brumlik, M., Cheng, P., Curiel, T., Myers, L., Lackner, A., Alvarez, X., Ochoa, A., Chen, L., Zou, W. B7-H4 expression identifies a novel suppressive macrophage population in human ovarian carcinoma. J. Exp. Med. 203: 871-881, 2006.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

96950 MW

Observed Molecular Weight

31 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

V-set domain containing T cell activation inhibitor 1

Subcellular Location

Cell membrane. Single-pass type I membrane protein.

Protein Name

Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2

Isotype

Rabbit IgG

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.