Anti-Aldolase/ALDOB Antibody Picoband®

About this product

Boster Bio Anti-Aldolase/ALDOB Antibody Picoband® catalog # A03893-2. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A03893-2

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Aldolase B also known as fructose-bisphosphate aldolase B or liver-type aldolase is one of three isoenzymes (A, B, and C) of the class I fructose 1,6-bisphosphate aldolase enzyme (EC 4.1.2.13), and plays a key role in both glycolysis and gluconeogenesis. It is mapped to 9q31.1. Fructose-1,6-bisphosphate aldolase (EC 4.1.2.13) is a tetrameric glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. Vertebrates have 3 aldolase isozymes which are distinguished by their electrophoretic and catalytic properties. Differences indicate that aldolases A, B, and C are distinct proteins, the products of a family of related 'housekeeping' genes exhibiting developmentally regulated expression of the different isozymes. The developing embryo produces aldolase A, which is produced in even greater amounts in adult muscle where it can be as much as 5% of total cellular protein. In adult liver, kidney and intestine, aldolase A expression is repressed and aldolase B is produced. In brain and other nervous tissue, aldolase A and C are expressed about equally. There is a high degree of homology between aldolase A and C. Defects in ALDOB cause hereditary fructose intolerance.

Synonyms

Fructose-bisphosphate aldolase B; Liver-type aldolase; ALDOB; ALDB

Gene Name

ALDOB

Gene ID

229

UniProt

P05062

Host

Rabbit

Reactivity

Human, Monkey, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human Aldolase/ALDOB recombinant protein (Position: Y85-Y364) .

Clonality

Polyclonal

Tissue Specificity

Pancreas-specific (at protein level) . Loss of expression is seen in ductal type pancreas cancers.

Applications

ELISA, Flow Cytometry, IF, IHC, ICC, WB

Field of Research

Cancer, Cell Biology, Cytoskeleton/ECM, ECM Enzymes, Extracellular Matrix, Invasion/Microenvironment, Proteolysis/Ubiquitin, Proteolytic Enzymes, Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Transcription factor implicated in the cell fate determination in various organs. Binds to the E-box consensus sequence 5'-CANNTG-3'. Plays a role in early and late pancreas development and differentiation. Important for determining whether cells allocated to the pancreatic buds continue towards pancreatic organogenesis or revert back to duodenal fates. May be involved in the maintenance of exocrine pancreas-specific gene expression including ELA1 and amylase. Required for the formation of pancreatic acinar and ductal cells. Plays an important role in cerebellar development. ly regulated by FOXN4 and RORC during retinal development, FOXN4-PTF1A pathway plays a central role in ing the differentiation of retinal progenitors towards horizontal and amacrine fates.

References & Citations

1. Dalby AR, Tolan DR, Littlechild JA (November 2001) . The structure of human liver fructose-1,6-bisphosphate aldolase. Acta Crystallogr. D. 57 (Pt 11) : 1526–33. 2. Henry I, Gallano P, Besmond C, Weil D, Mattei MG, Turleau C, Boué J, Kahn A, Junien C (July 1985) . The structural gene for aldolase B (ALDB) maps to 9q13----32. Ann. Hum. Genet. 49 (Pt 3) : 173–80. 3. Sygusch J, Beaudry D, Allaire M (November 1987) . Molecular architecture of rabbit skeletal muscle aldolase at 2.7-A resolution. Proc. Natl. Acad. Sci. U.S.A. 84 (22) : 7846–50.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Observed Molecular Weight

39 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

Aldolase, fructose-bisphosphate B

Subcellular Location

Nucleus. Cytoplasm.

Isotype

Rabbit IgG

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.