Anti-CEBP Alpha/CEBPA Antibody Picoband®

About this product

Boster Bio Anti-CEBP Alpha/CEBPA Antibody Picoband® catalog # A00386-1. Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A00386-1

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

CEBPA, CCAAT/enhancer-binding protein alpha is a protein that in humans is encoded by the CEBPA gene. The CEBPA gene is intronless. Using human/hamster somatic cell hybrids containing restricted fragments of human chromosome 19, the CEBPA gene is mapped to chromosome 19q13.1, between the GPI and TGFB1 genes. The protein encoded by this intronless gene is a bZIP transcription factor which can bind as a homodimer to certain promoters and enhancers. It can also form heterodimers with the related proteins CEBP-beta and CEBP-gamma. The encoded protein has been shown to bind to the promoter and modulate the expression of the gene encoding leptin, a protein that plays an important role in body weight homeostasis.

Synonyms

CCAAT/enhancer-binding protein alpha; C/EBP alpha; CEBPA; CEBP

Gene Name

CEBPA

Gene ID

1050

UniProt

P49715

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human CEBP Alpha/CEBPA recombinant protein (Position: Y138-Q221) .

Clonality

Polyclonal

Tissue Specificity

Expressed in fetal brain.

Applications

ELISA, Flow Cytometry, IF, ICC, WB

Field of Research

Cell Biology, Proteasome / Ubiquitin, Proteolysis/Ubiquitin

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Transcription factor that coordinates proliferation arrest and the differentiation of myeloid progenitors, adipocytes, hepatocytes, and cells of the lung and the placenta. Binds ly to the consensus DNA sequence 5'-T[TG]NNGNAA[TG]-3' acting as an activator on distinct target genes (PubMed:11242107) . During early embryogenesis, plays essential and redundant functions with CEBPB. Essential for the transition from common myeloid progenitors (CMP) to granulocyte/monocyte progenitors (GMP) . Critical for the proper development of the liver and the lung (By similarity) . Necessary for terminal adipocyte differentiation, is required for postnatal maintenance of systemic energy homeostasis and lipid storage (By similarity) . To regulate these different processes at the proper moment and tissue, interplays with other transcription factors and modulators. Downregulates the expression of genes that maintain cells in an undifferentiated and proliferative state through E2F1 repression, which is critical for its ability to induce adipocyte and granulocyte terminal differentiation. Reciprocally E2F1 blocks adipocyte differentiation by binding to specific promoters and repressing CEBPA binding to its target gene promoters. Proliferation arrest also depends on a functional binding to SWI/SNF complex (PubMed:14660596) . In liver, regulates gluconeogenesis and lipogenesis through different mechanisms. To regulate gluconeogenesis, functionally cooperates with FOXO1 binding to IRE-controlled promoters and regulating the expression of target genes such as PCK1 or G6PC. To modulate lipogenesis, interacts and transcriptionally synergizes with SREBF1 in promoter activation of specific lipogenic target genes such as ACAS2. In adipose tissue, seems to act as FOXO1 coactivator accessing to ADIPOQ promoter through FOXO1 binding sites (By similarity) .

References & Citations

1. Helbling, D., Mueller, B. U., Timchenko, N. A., Hagemeijer, A., Jotterand, M., Meyer-Monard, S., Lister, A., Rowley, J. D., Huegli, B., Fey, M. F., Pabst, T.The leukemic fusion gene AML1-MDS1-EVI1 suppresses CEBPA in acute myeloid leukemia by activation of calreticulin.Proc. Nat. Acad. Sci. 101: 13312-13317, 2004. 2. Hendricks-Taylor, L. R., Bachinski, L. L., Siciliano, M. J., Fertitta, A., Trask, B., de Jong, P. J., Ledbetter, D. H., Darlington, G. J.The CCAAT/enhancer binding protein (C/EBP-alpha) gene (CEBPA) maps to human chromosome 19q13.1 and the related nuclear factor NF-IL6 (C/EBP-beta) gene (CEBPB) maps to human chromosome 20q13.1.Genomics 14: 12-17, 1992. 3. Swart, G. W. M., van Groningen, J. J. M., van Ruissen, F., Bergers, M., Schalkwijk, J.Transcription factor C/EBP-alpha: novel sites of expression and cloning of the human gene.Biol. Chem. 378: 373-379, 1997.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Observed Molecular Weight

42 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

CCAAT enhancer binding protein alpha

Subcellular Location

Nucleus.

Isotype

Rabbit IgG

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.