Anti-CD35/CR1 Antibody Picoband®

About this product

Boster Bio Anti-CD35/CR1 Antibody Picoband® catalog # A01022-1. Tested in ELISA, IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A01022-1

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Complement receptor type 1 (CR1) also known as C3b/C4b receptor or CD35 (cluster of differentiation 35) is a protein that in humans is encoded by the CR1 gene. This gene is a member of the receptors of complement activation (RCA) family and is located in the 'cluster RCA' region of chromosome 1. The gene encodes a monomeric single-pass type I membrane glycoprotein found on erythrocytes, leukocytes, glomerular podocytes, and splenic follicular dendritic cells. The Knops blood group system is a system of antigens located on this protein. The protein mediates cellular binding to particles and immune complexes that have activated complement. Decreases in expression of this protein and/or mutations in its gene have been associated with gallbladder carcinomas, mesangiocapillary glomerulonephritis, systemic lupus erythematosus and sarcoidosis. Mutations in this gene have also been associated with a reduction in Plasmodium falciparum rosetting, conferring protection against severe malaria. Alternate allele-specific splice variants, encoding different isoforms, have been characterized. Additional allele specific isoforms, including a secreted form, have been described but have not been fully characterized.

Synonyms

Complement receptor type 1; C3b/C4b receptor; CD35; CR1; C3BR

Gene Name

CR1

Gene ID

1378

UniProt

P17927

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human CD35/CR1 recombinant protein (Position: D109-Q957) .

Clonality

Polyclonal

Tissue Specificity

Present on erythrocytes, a subset of T cells, mature B cells, follicular dendritic cells, monocytes and granulocytes.

Applications

ELISA, IHC, WB

Field of Research

Cytokines, Immunology, Innate Immunity, Interleukins, Protein Phosphorylation, Ser/Thr Kinases, Signal Transduction, TLR Signaling, TNF Superfamily

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Membrane immune adherence receptor that plays a critical role in the capture and clearance of complement-opsonized pathogens by erythrocytes and monocytes/macrophages (PubMed:2963069) . Mediates the binding by these cells of particles and immune complexes that have activated complement to eliminate them from the circulation (PubMed:2963069) . Acts also in the inhibition of spontaneous complement activation by impairing the formation and function of the alternative and classical pathway C3/C5 convertases, and by serving as a cofactor for the cleavage by factor I of C3b to iC3b, C3c and C3d, g, and of C4b to C4c and C4d (PubMed:2972794, PubMed:8175757) . Plays also a role in immune regulation by contributing, upon ligand binding, to the generation of regulatory T cells from activated helper T cells (PubMed:25742728) . (Microbial infection) Acts as a receptor for Epstein-Barr virus.

References & Citations

1. Jacobson AC, Weis JH (Sep 2008) . Comparative functional evolution of human and mouse CR1 and CR2. J. Immunol. 181 (5) : 2953–9. 2. Khera R, Das N (February 2009) . Complement Receptor 1: Disease associations and therapeutic implications. Molecular Immunology. 46 (5) : 761–772. 3. Moulds JM, Nickells MW, Moulds JJ, Brown MC, Atkinson JP (May 1991) . The C3b/C4b receptor is recognized by the Knops, McCoy, Swain-langley, and York blood group antisera. J. Exp. Med. 173 (5) : 1159–63.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

47579 MW

Observed Molecular Weight

250 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Complement C3b/C4b receptor 1 (Knops blood group)

Subcellular Location

Cytoplasm.

Protein Name

2',3'-cyclic-nucleotide 3'-phosphodiesterase

Isotype

Rabbit IgG

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.