- Catalog number
- A00962-2
- Size / count
- 100 µg/Vial
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Forkhead box C1, also known as?FOXC1, is a?protein?which in humans is encoded by the?FOXC1?gene. It is mapped to 6p25.3. This gene belongs to the forkhead family of transcription factors which is characterized by a distinct DNA-binding forkhead domain. The specific function of this gene has not yet been determined; however, it has been shown to play a role in the regulation of embryonic and ocular development. Mutations in this gene cause various glaucoma phenotypes including primary congenital glaucoma, autosomal dominant iridogoniodysgenesis anomaly, and Axenfeld-Rieger anomaly.
- Synonyms
- Forkhead box protein C1; Forkhead-related protein FKHL7; Forkhead-related transcription factor 3; FREAC-3; FOXC1; FKHL7, FREAC3
- Gene Name
- FOXC1
- Gene ID
- 2296
- UniProt
- Q12948
- Host
- Rabbit
- Reactivity
- Human
- Cross Reactivity
- No cross-reactivity with other proteins.
- Immunogen
- E.coli-derived human FOXC1 recombinant protein (Position: T392-F554) .
- Clonality
- Polyclonal
- Tissue Specificity
- Expressed in keratinocytes of epidermis and hair follicle (PubMed:27907090) . Expressed strongly in microvascular invasion (MVI) formation, basal-like breast cancer (BLBC) and hepatocellular tumors (PubMed:20406990, PubMed:22991501) . Expressed in breast cancers (at protein level) (PubMed:26565916) . Expressed in hematopoietic cells (PubMed:8499623) .
- Applications
- ELISA, IF, ICC, WB
- Field of Research
- Apoptosis, Atherosclerosis, Cardiovascular, Caspases, Cell Biology, Cell Type Markers, Intracellular, Neural Stem Cells, Stem Cells, Tags & Cell Markers, Tumor Associated
- Purification
- Immunogen affinity purified.
- Concentration
- Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
- Form
- Lyophilized
- Reconstitution
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
- Function
- DNA-binding transcriptional factor that plays a role in a broad range of cellular and developmental processes such as eye, bones, cardiovascular, kidney and skin development (PubMed:11782474, PubMed:15299087, PubMed:15684392, PubMed:16492674, PubMed:27907090, PubMed:14506133, PubMed:14578375, PubMed:15277473, PubMed:16449236, PubMed:17210863, PubMed:19793056, PubMed:19279310, PubMed:25786029, PubMed:27804176) . Acts either as a transcriptional activator or repressor (PubMed:11782474) . Binds to the consensus binding site 5'-[G/C][A/T]AAA[T/C]AA[A/C]-3' in promoter of target genes (PubMed:7957066, PubMed:11782474, PubMed:12533514, PubMed:14506133, PubMed:19793056, PubMed:27804176) . Upon DNA-binding, promotes DNA bending (PubMed:7957066, PubMed:14506133) . Acts as a transcriptional coactivator (PubMed:26565916) . Stimulates Indian hedgehog (Ihh) -induced target gene expression mediated by the transcription factor GLI2, and hence regulates endochondral ossification (By similarity) . Acts also as a transcriptional coregulator by increasing DNA-binding capacity of GLI2 in breast cancer cells (PubMed:26565916) . Regulates FOXO1 through binding to a conserved element, 5'-GTAAACAAA-3' in its promoter region, implicating FOXC1 as an important regulator of cell viability and resistance to oxidative stress in the eye (PubMed:17993506) . Cooperates with transcription factor FOXC2 in regulating expression of genes that maintain podocyte integrity (By similarity) . Promotes cell growth inhibition by stopping the cell cycle in the G1 phase through TGFB1-mediated signals (PubMed:12408963) . Involved in epithelial-mesenchymal transition (EMT) induction by increasing cell proliferation, migration and invasion (PubMed:20406990, PubMed:22991501) . Involved in chemokine CXCL12-induced endothelial cell migration through the control of CXCR4 expression (By similarity) . Plays a role in the gene regulatory network essential for epidermal keratinocyte terminal differentiation (PubMed:27907090) . Essential developmental transcriptional factor required for mesoderm-derived tissues, such as the somites, skin, bone and cartilage. Positively regulates CXCL12 and stem cell factor expression in bone marrow mesenchymal progenitor cells, and hence plays a role in the development and maintenance of mesenchymal niches for haematopoietic stem and progenitor cells (HSPC) . Plays a role in corneal transparency by preventing both blood vessel and lymphatic vessel growth during embryonic development in a VEGF-dependent manner. Involved in chemokine CXCL12-induced endothelial cell migration through the control of CXCR4 expression (By similarity) . May function as a tumor suppressor (PubMed:12408963) .
- References & Citations
- 1. Pierrou S, Hellqvist M, Samuelsson L, Enerbäck S, Carlsson P (October 1994) . Cloning and characterization of seven human forkhead proteins: binding site specificity and DNA bending. The EMBO Journal. 13 (20) : 5002–12. 2. Nishimura DY, Swiderski RE, Alward WL, Searby CC, Patil SR, Bennet SR, Kanis AB, Gastier JM, Stone EM, Sheffield VC (June 1998) . The forkhead transcription factor gene FKHL7 is responsible for glaucoma phenotypes which map to 6p25. Nature Genetics. 19 (2) : 140–7. 3. Kume T, Jiang H, Topczewska JM, Hogan BL (September 2001) . The murine winged helix transcription factors, Foxc1 and Foxc2, are both required for cardiovascular development and somitogenesis. Genes & Development. 15 (18) : 2470–82. doi:10.1101/gad.907301.
- Storage Conditions
- Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
- Calculated Molecular Weight
- 53 kDa
- Observed Molecular Weight
- 75 kDa
- Specificity
- No cross reactivity with other proteins.
- Gene Name Synonym
- Forkhead box C1
- Isotype
- Rabbit IgG
- Contents
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
