Anti-RPL13A Antibody Picoband®

About this product

Boster Bio Anti-RPL13A Antibody Picoband® catalog # A03571-1. Tested in ELISA, IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A03571-1

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

60S ribosomal protein L13a?is a?protein?that in humans is encoded by the?RPL13A?gene. It is mapped to 19q13.33. Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a member of the L13P family of ribosomal proteins that is a component of the 60S subunit. The encoded protein also plays a role in the repression of inflammatory genes as a component of the IFN-gamma-activated inhibitor of translation (GAIT) complex. This gene is co-transcribed with the small nucleolar RNA genes U32, U33, U34, and U35, which are located in the second, fourth, fifth, and sixth introns, respectively. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed throughout the genome. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

Synonyms

60S ribosomal protein L13a; 23 kDa highly basic protein; Large ribosomal subunit protein Ul13; RPL13A

Gene Name

RPL13A

Gene ID

23521

UniProt

P40429

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human RPL13A recombinant protein (Position:A2-V203) .

Clonality

Polyclonal

Applications

ELISA, IHC, WB

Field of Research

Epigenetics and Nuclear Signaling, Mediator Complex, Transcription

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Associated with ribosomes but is not required for canonical ribosome function and has extra-ribosomal functions. Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma activation and subsequent phosphorylation dissociates from the ribosome and assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation. In the GAIT complex interacts with m7G cap-bound eIF4G at or near the eIF3-binding site and blocks the recruitment of the 43S ribosomal complex. Involved in methylation of rRNA.

References & Citations

1. Wool IG, Chan YL, Glück A (1996) . Structure and evolution of mammalian ribosomal proteins. Biochem. Cell Biol. 73 (11–12) : 933–947. 2. Price SR, Nightingale MS, Bobak DA, et al. (1993) . Conservation of a 23-kDa human transplantation antigen in mammalian species. Genomics. 14 (4) : 959–964. 3. Nicoloso M, Qu LH, Michot B, Bachellerie JP (1996) . Intron-encoded, antisense small nucleolar RNAs: the characterization of nine novel species points to their role as guides for the 2'-O-ribose methylation of rRNAs. J. Mol. Biol. 260 (2) : 178–195.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Observed Molecular Weight

24 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Ribosomal protein L13a

Isotype

Rabbit IgG

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.