Anti-Osteoprotegerin/TNFRSF11B Antibody Picoband®

About this product

Boster Bio Anti-Osteoprotegerin/TNFRSF11B Antibody Picoband® catalog # A00863. Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A00863

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Tumor necrosis factor receptor superfamily member 11B (TNFRSF11B), also known as OPG, is a protein that in humans is encoded by the TNFRSF11B gene. OPG is a cytokine receptor, and a member of the tumor necrosis factor (TNF) receptor superfamily. By analysis of radiation hybrids, TNFRSF11B gene was mapped to chromosome 8q24. OPG is a decoy receptor for the receptor activator of nuclear factor kappa B ligand (RANKL) . By binding RANKL, OPG inhibits nuclear kappa B (NF-κB) which is a central and rapid acting transcription factor for immune-related genes, and a key regulator of inflammation, innate immunity, and cell survival and differentiation. OPG binding to RANKL on osteoblast/stromal cells, blocks the RANKL-RANK ligand interaction between osteoblast/stromal cells and ostepclast precursors.

Synonyms

Tumor necrosis factor receptor superfamily member 11B; Osteoclastogenesis inhibitory factor; Osteoprotegerin; TNFRSF11B; OCIF, OPG

Gene Name

TNFRSF11B

Gene ID

4982

UniProt

O00300

Host

Rabbit

Reactivity

Human, Monkey, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human Osteoprotegerin/TNFRSF11B recombinant protein (Position: Q247-R296) .

Clonality

Polyclonal

Tissue Specificity

Highly expressed in adult lung, heart, kidney, liver, spleen, thymus, prostate, ovary, small intestine, thyroid, lymph node, trachea, adrenal gland, testis, and bone marrow. Detected at very low levels in brain, placenta and skeletal muscle. Highly expressed in fetal kidney, liver and lung.

Applications

ELISA, Flow Cytometry, IHC, WB

Field of Research

Angiogenesis, Cancer, Cardiovascular, Cell Biology, Cell Cycle, Cell Cycle Inhibitors, Cytoskeleton/ECM, Developmental Biology, Extracellular Matrix, Lineage Markers, Lineage Specification, Mesenchymal Stem Cells, Protein Phosphorylation, Receptor Tyrosine Kinases, Signal Transduction, Stem Cells, Structures, Tyrosine Kinases

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Acts as decoy receptor for TNFSF11/RANKL and thereby neutralizes its function in osteoclastogenesis. Inhibits the activation of osteoclasts and promotes osteoclast apoptosis in vitro. Bone homeostasis seems to depend on the local ratio between TNFSF11 and TNFRSF11B. May also play a role in preventing arterial calcification. May act as decoy receptor for TNFSF10/TRAIL and protect against apoptosis. TNFSF10/TRAIL binding blocks the inhibition of osteoclastogenesis.

References & Citations

1. Krakauer T (2008) . Nuclear factor-kappa B: fine-tuning a central integrator of diverse biologic stimuli. Int. Rev. Immunol. 27 (5) : 286–92. 2. Khosla S (December 2001) . Minireview: the OPG/RANKL/RANK system. Endocrinology 142 (12) : 5050–5. 3. Schoppet M, Preissner KT, Hofbauer LC (April 2002) . RANK ligand and osteoprotegerin: paracrine regulators of bone metabolism and vascular function. Arterioscler. Thromb. Vasc. Biol. 22 (4) : 549–53.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

34632 MW

Observed Molecular Weight

55 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

TNF receptor superfamily member 11b

Subcellular Location

Secreted, extracellular space, extracellular matrix, basement membrane.

Protein Name

SPARC

Isotype

Rabbit IgG

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.