Anti-HOMER3 Antibody Picoband® (monoclonal, 9G13)

About this product

Boster Bio Anti-HOMER3 Antibody Picoband® (monoclonal, 9G13) catalog # M09145. Tested in Flow Cytometry, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

M09145

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Homer protein homolog 3 is a protein that in humans is encoded by the HOMER3 gene. This gene encodes a member of the HOMER family of postsynaptic density scaffolding proteins that share a similar domain structure consisting of an N-terminal Enabled/vasodilator-stimulated phosphoprotein homology 1 domain which mediates protein-protein interactions, and a carboxy-terminal coiled-coil domain and two leucine zipper motifs that are involved in self-oligomerization. The encoded protein binds numerous other proteins including group I metabotropic glutamate receptors, inositol 1,4,5-trisphosphate receptors and amyloid precursor proteins and has been implicated in diverse biological functions such as neuronal signaling, T-cell activation and trafficking of amyloid beta peptides.

Synonyms

Homer protein homolog 3; Homer-3; HOMER3

Gene Name

HOMER3

Gene ID

9454

UniProt

Q9NSC5

Host

Mouse

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E. coli-derived human HOMER3 recombinant protein (Position: R282-A360) . Human HOMER3 shares 88.6% and 89.9% amino acid (aa) sequence identity with mouse and rat HOMER3, respectively.

Clonality

Monoclonal

Clone

Clone: 9G13

Tissue Specificity

Ubiquitously expressed with highest levels in spleen, thymus and immature brain.

Applications

Flow Cytometry, WB

Field of Research

Chaperones, Protein Trafficking, Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500μg/ml.

Function

Postsynaptic density scaffolding protein. Binds and cross-links cytoplasmic regions of GRM1, GRM5, ITPR1, DNM3, RYR1, RYR2, SHANK1 and SHANK3. By physically linking GRM1 and GRM5 with ER-associated ITPR1 receptors, it aids the coupling of surface receptors to intracellular calcium release. Isoforms can be differently regulated and may play an important role in maintaining the plasticity at glutamatergic synapses. Negatively regulates T cell activation by inhibiting the calcineurin-NFAT pathway. Acts by competing with calcineurin/PPP3CA for NFAT protein binding, hence preventing NFAT activation by PPP3CA

References & Citations

1. Soloviev MM, Ciruela F, Chan WY, McIlhinney RA (Feb 2000) . Molecular characterisation of two structurally distinct groups of human homers, generated by extensive alternative splicing. Journal of Molecular Biology. 295 (5) : 1185–200. 2. Entrez Gene: HOMER3 homer homolog 3 (Drosophila) .

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Observed Molecular Weight

45 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

Homer scaffold protein 3

Subcellular Location

Postsynaptic density; Cytoplasm; synapse

Isotype

Mouse IgG2a

Contents

243