Anti-CD3 epsilon/Cd3e Antibody Picoband®

About this product

Boster Bio Anti-CD3 epsilon/Cd3e Antibody Picoband® catalog # A02765-1. Tested in ELISA, Flow Cytometry , IHC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A02765-1

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

CD3e molecule, epsilon also known as CD3E is a polypeptide which in humans is encoded by the CD3E gene which resides on chromosome 11. It is mapped to 11q23.3. The protein encoded by this gene is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development.

Synonyms

T-cell surface glycoprotein CD3 epsilon chain; T-cell surface antigen T3/Leu-4 epsilon chain; CD3e; Cd3e

Gene Name

Cd3e

Gene ID

12501

UniProt

P22646

Host

Rabbit

Reactivity

Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived mouse CD3 epsilon/Cd3e recombinant protein (Position: D22-A113) .

Clonality

Polyclonal

Applications

ELISA, IHC, WB, Flow Cytometry

Field of Research

Cardiovascular, Chemokines, Immunology, Innate Immunity, Platelets

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways. In addition of this role of signal transduction in T-cell activation, CD3E plays an essential role in correct T-cell development (PubMed:19956738, PubMed:24899501) . Participates also in internalization and cell surface down-regulation of TCR-CD3 complexes via endocytosis sequences present in CD3E cytosolic region.

References & Citations

1. Clevers HC, Dunlap S, Wileman TE, Terhorst C (November 1988) .Human CD3-epsilon gene contains three miniexons and is transcribed from a non-TATA promoter. Proc. Natl. Acad. Sci. U.S.A. 85 (21) : 8156–60. 2. Entrez Gene: CD3E CD3e molecule, epsilon (CD3-TCR complex) . 3. Gold DP, Puck JM, Pettey CL, Cho M, Coligan J, Woody JN, Terhorst C (1986) . Isolation of cDNA clones encoding the 20K non-glycosylated polypeptide chain of the human T-cell receptor/T3 complex. Nature 321 (6068) : 431–4.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

12252 MW

Observed Molecular Weight

23 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

CD3 antigen, epsilon polypeptide

Subcellular Location

Cell membrane; Single-pass type I membrane protein

Isotype

Rabbit IgG

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.