Anti-Cytokeratin 20/Krt20 Antibody Picoband®

About this product

Boster Bio Anti-Cytokeratin 20/Krt20 Antibody Picoband® catalog # A02828-2. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A02828-2

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Keratin 20, often abbreviated CK20, is a protein that in humans is encoded by the KRT20 gene. This gene encodes a member of the keratin protein family and is found within a cluster of cytokeratin genes on chromosome 11. Keratins are cytoskeletal proteins that are preferentially expressed in epithelial cells. The encoded protein may help maintain intermediate filament organization in intestinal epithelium. Phosphorylation of this protein may also influence mucin secretion in the small intestine.

Synonyms

Keratin, type I cytoskeletal 20; Cytokeratin-20; CK-20; Keratin-20; K20; Krt20

Gene Name

Krt20

Gene ID

66809

UniProt

Q9D312

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived mouse Cytokeratin 20/Krt20 recombinant protein (Position: R29-V431) .

Clonality

Polyclonal

Tissue Specificity

Expressed at low levels in the more differentiated suprabasal regions of the small intestine, and at higher levels in the colon, mainly in the upper region and in scattered cells throughout the remaining epithelium. Also expressed in epithelial cells of bladder, ileum and stomach and at lower levels in pancreas and earskin. The phosphorylated form is nearly exclusively expressed in goblet cells of the small intestine and in the lumen-proximal cells of the colon (at protein level) . Also expressed in jejunum and duodenum.

Applications

ELISA, Flow Cytometry, IF, IHC, ICC, WB

Field of Research

Cell Biology, Co-Activators/Co-Repressors, Epigenetics and Nuclear Signaling, Nuclear Receptors, Nuclear Signaling Pathways, Proteasome / Ubiquitin, Proteolysis/Ubiquitin, Transcription, Transcription Factors, Ubiquitin & Ubiquitin Like Modifiers

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Plays a significant role in maintaining keratin filament organization in intestinal epithelia. When phosphorylated, plays a role in the secretion of mucin in the small intestine.

References & Citations

1. Calnek, D., Quaroni, A. Differential localization by in situ hybridization of distinct keratin mRNA species during intestinal epithelial cell development and differentiation. Differentiation 53: 95-104, 1993. 2. Moll, R., Schiller, D. L., Franke, W. W. Identification of protein IT of the intestinal cytoskeleton as a novel type I cytokeratin with unusual properties and expression patterns. J. Cell Biol. 111: 567-580, 1990. 3. Rogers, M. A., Langbein, L., Winter, H., Ehmann, C., Praetzel, S., Korn, B., Schweizer, J. Characterization of a cluster of human high/ultrahigh sulfur keratin-associated protein genes embedded in the type I keratin gene domain on chromosome 17q12-21. J. Biol. Chem. 276: 19440-19451, 2001.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

96950 MW

Observed Molecular Weight

48 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

Keratin 20

Subcellular Location

Cytoplasm.

Protein Name

Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2

Isotype

Rabbit IgG

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.