Anti-c-Maf/MAF Antibody Picoband®

About this product

Boster Bio Anti-c-Maf/MAF Antibody Picoband® catalog # A00654-1. Tested in ICC/IF, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A00654-1

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Transcription factor Maf also known as proto-oncogene c-Maf or V-maf musculoaponeurotic fibrosarcoma oncogene homolog is a transcription factor that in humans is encoded by the MAF gene. It is mapped to 16q23.2. The protein encoded by this gene is a DNA-binding, leucine zipper-containing transcription factor that acts as a homodimer or as a heterodimer. Depending on the binding site and binding partner, the encoded protein can be a transcriptional activator or repressor. This protein plays a role in the regulation of several cellular processes, including embryonic lens fiber cell development, increased T-cell susceptibility to apoptosis, and chondrocyte terminal differentiation. Defects in this gene are a cause of juvenile-onset pulverulent cataract as well as congenital cerulean cataract 4 (CCA4) . Two transcript variants encoding different isoforms have been found for this gene.

Synonyms

Transcription factor Maf; Proto-oncogene c-Maf; V-maf musculoaponeurotic fibrosarcoma oncogene homolog; MAF

Gene Name

MAF

Gene ID

4094

UniProt

O75444

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human c-Maf/MAF, identical to the related mouse and rat sequences.

Clonality

Polyclonal

Tissue Specificity

Expressed in endothelial cells.

Applications

ICC/IF, WB

Field of Research

Adaptive Immunity, Cardiovascular, Hematopoietic Progenitors, Immunology, Lymphoid, Myeloid, Stem Cells, T Cells, T Lymphocytic Lineage

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Acts as a transcriptional activator or repressor. Involved in embryonic lens fiber cell development. Recruits the transcriptional coactivators CREBBP and/or EP300 to crystallin promoters leading to up-regulation of crystallin gene during lens fiber cell differentiation. Activates the expression of IL4 in T helper 2 (Th2) cells. Increases T-cell susceptibility to apoptosis by interacting with MYB and decreasing BCL2 expression. Together with PAX6, transactivates strongly the glucagon gene promoter through the G1 element. Activates transcription of the CD13 proximal promoter in endothelial cells. Represses transcription of the CD13 promoter in early stages of myelopoiesis by affecting the ETS1 and MYB cooperative interaction. Involved in the initial chondrocyte terminal differentiation and the disappearance of hypertrophic chondrocytes during endochondral bone development. Binds to the sequence 5'-[GT]G[GC]N[GT]NCTCAGNN-3' in the L7 promoter. Binds to the T-MARE (Maf response element) sites of lens-specific alpha- and beta-crystallin gene promoters. Binds element G1 on the glucagon promoter. Binds an AT-rich region adjacent to the TGC motif (atypical Maf response element) in the CD13 proximal promoter in endothelial cells (By similarity) . When overexpressed, represses anti-oxidant response element (ARE) -mediated transcription. Involved either as an oncogene or as a tumor suppressor, depending on the cell context. Binds to the ARE sites of detoxifying enzyme gene promoters.

References & Citations

1. Nishizawa M, Kataoka K, Goto N, Fujiwara KT, Kawai S (Oct 1989) . v-maf, a viral oncogene that encodes a leucine zipper motif. Proceedings of the National Academy of Sciences of the United States of America. 86 (20) : 7711–5. 2. Olbrot M, Rud J, Moss LG, Sharma A (May 2002) . Identification of beta-cell-specific insulin gene transcription factor RIPE3b1 as mammalian MafA. Proceedings of the National Academy of Sciences of the United States of America. 99 (10) : 6737–42. 3. Melloul D, Marshak S, Cerasi E (Mar 2002) . Regulation of insulin gene transcription. Diabetologia. 45 (3) : 309–26.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

39 kDa

Observed Molecular Weight

52 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

MAF bZIP transcription factor

Subcellular Location

Membrane; Single-pass type I membrane protein.

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.