Anti-Neurofibromin/NF1 Antibody Picoband®

About this product

Boster Bio Anti-Neurofibromin/NF1 Antibody Picoband® catalog # A00043-1. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A00043-1

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Neurofibromin 1 (NF1) is a gene in humans that is located on chromosome 17. This gene product appears to function as a negative regulator of the ras signal transduction pathway. Mutations in this gene have been linked to neurofibromatosis type 1, juvenile myelomonocytic leukemia and Watson syndrome. The mRNA for this gene is subject to RNA editing (CGA>UGA->Arg1306Term) resulting in premature translation termination. Alternatively spliced transcript variants encoding different isoforms have also been described for this gene.

Synonyms

Neurofibromin; Neurofibromatosis-related protein NF-1; Neurofibromin truncated; NF1

Gene Name

NF1

Gene ID

4763

UniProt

P21359

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human Neurofibromin/NF1 recombinant protein (Position: R160-Q270) .

Clonality

Polyclonal

Tissue Specificity

Detected in brain, peripheral nerve, lung, colon and muscle.

Applications

ELISA, Flow Cytometry, IF, IHC, ICC, WB

Field of Research

Apoptosis, Apoptotic Markers, Cancer, Caspases, Cell Biology, Cell Death, Intracellular, Invasion/Microenvironment, Metabolism, Metabolism Processes, Pathways and Processes, Proteolysis/Ubiquitin, Proteolytic Enzymes

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Stimulates the GTPase activity of Ras. NF1 shows greater affinity for Ras GAP, but lower specific activity. May be a regulator of Ras activity.

References & Citations

1. Scheffzek K, Welti S (2012) . Neurofibromin: Protein Domains and Functional Characteristics. In Upadhyaya M, Cooper D (eds.) . Neurofibromatosis Type 1. Berlin, Heidelberg: Springer. pp. 305–326. 2. Trovó-Marqui AB, Tajara EH (July 2006) . Neurofibromin: a general outlook. Clinical Genetics. 70 (1) : 1–13. 3. Peltonen S, Pöyhönen M (2012) . Clinical Diagnosis and Atypical Forms of NF1. In Upadhyaya M, Cooper D (eds.) . Neurofibromatosis Type 1. Berlin, Heidelberg: Springer. pp. 17–30.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Observed Molecular Weight

319 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

Neurofibromin 1

Subcellular Location

Cytoplasm.

Isotype

Rabbit IgG

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.