Anti-Cytokeratin 18 KRT18 Antibody Picoband® (monoclonal, 7I6)

About this product

Boster Bio Anti-Cytokeratin 18 KRT18 Antibody Picoband® (monoclonal, 7I6) catalog # M01357-4. Tested in Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

M01357-4

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Keratin 18, mapped to 12q13.13, is a type I cytokeratin. It is, together with its filament partner keratin 8, perhaps the most commonly found products of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene.

Synonyms

Keratin, type I cytoskeletal 18; Cell proliferation-inducing gene 46 protein; Cytokeratin-18; CK-18; Keratin-18; K18; KRT18; CYK18; PIG46

Gene Name

KRT18

Gene ID

3875

UniProt

P05783

Host

Mouse

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human Cytokeratin 18 recombinant protein (Position: E204-H430) . Human Cytokeratin 18 shares 87.7% and 85.9% amino acid (aa) sequence identity with mouse and rat Cytokeratin 18, respectively.

Clonality

Monoclonal

Clone

Clone: 7I6

Tissue Specificity

Expressed in colon, placenta, liver and very weakly in exocervix. Increased expression observed in lymph nodes of breast carcinoma.

Applications

Flow Cytometry, IF, IHC, IHC-F, ICC, WB

Field of Research

DNA/RNA, DNA Damage & Repair, DNA Damage Response, DNA Synthesis, Epigenetics and Nuclear Signaling

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500μg/ml.

Function

Involved in the uptake of thrombin-antithrombin complexes by hepatic cells. When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6) -mediated barrier protection.

References & Citations

1. Entrez Gene: KRT18 keratin 18. 2. Ku, N.-O., Wright, T. L., Terrault, N. A., Gish, R., Omary, M. B. Mutation of human keratin 18 in association with cryptogenic cirrhosis. J. Clin. Invest. 99: 19-23, 1997. 3. Ku, N.-O., Darling, J. M., Krams, S. M., Esquivel, C. O., Keeffe, E. B., Sibley, R. K., Lee, Y. M., Wright, T. L., Omary, M. B. Keratin 8 and 18 mutations are risk factors for developing liver disease of multiple etiologies. Proc. Nat. Acad. Sci. 100: 6063-6068, 2003.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Observed Molecular Weight

48 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

Keratin 18

Subcellular Location

Nucleolus. Perinuclear region.

Protein Name

Keratin, type I cytoskeletal 18

Isotype

Mouse IgG1

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.