Anti-ATF1 Antibody Picoband® (monoclonal, 7F8)

About this product

Boster Bio Anti-ATF1 Antibody Picoband® (monoclonal, 7F8) catalog # M01600-1. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

M01600-1

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

ATF1, also known as activating transcription factor 1, is a protein that in humans is encoded by the ATF1 gene. It is mapped to 12q13.12. This gene encodes an activating transcription factor, which belongs to the ATF subfamily and bZIP (basic-region leucine zipper) family. It influences cellular physiologic processes by regulating the expression of downstream target genes, which are related to growth, survival, and other cellular activities. This protein is phosphorylated at serine 63 in its kinase-inducible domain by serine/threonine kinases, cAMP-dependent protein kinase A, calmodulin-dependent protein kinase I/II, mitogen- and stress-activated protein kinase and cyclin-dependent kinase 3 (cdk-3) . Its phosphorylation enhances its transactivation and transcriptional activities, and enhances cell transformation.

Synonyms

Cyclic AMP-dependent transcription factor ATF-1; cAMP-dependent transcription factor ATF-1; Activating transcription factor 1; Protein TREB36; ATF1

Gene Name

ATF1

Gene ID

466

UniProt

P18846

Host

Mouse

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human ATF1 recombinant protein (Position: M1-V271) . Human ATF1 shares 91% amino acid (aa) sequence identity with mouse ATF1.

Clonality

Monoclonal

Clone

Clone: 7F8

Tissue Specificity

B-lymphocytes.

Applications

Flow Cytometry, IF, IHC, ICC, WB

Field of Research

Actin Binding Proteins, Actin, etc., Cytoskeleton, Cytoskeleton/ECM, Microfilaments, Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500μg/ml.

Function

Mediates B-cell B-cell interactions. May be involved in the localization of B-cells in lymphoid tissues. Binds sialylated glycoproteins; one of which is CD45. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site can be masked by cis interactions with sialic acids on the same cell surface. Upon ligand induced tyrosine phosphorylation in the immune response seems to be involved in regulation of B-cell antigen receptor signaling. Plays a role in positive regulation through interaction with Src family tyrosine kinases and may also act as an inhibitory receptor by recruiting cytoplasmic phosphatases via their SH2 domains that block signal transduction through dephosphorylation of signaling molecules.

References & Citations

1. ucman, J., Delattre, O., Desmaze, C., Epstein, A. L., Stenman, G., Speleman, F., Fletchers, C. D. M., Aurias, A., Thomas, G. EWS and ATF-1 gene fusion induced by t (12;22) translocation in malignant melanoma of soft parts. Nature Genet. 4: 341-345, 1993. 2. Entrez Gene: ATF1 activating transcription factor 1

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Observed Molecular Weight

38 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

Cyclic AMP-dependent transcription factor ATF-1

Subcellular Location

Cell membrane; Single-pass type I membrane protein

Protein Name

Activating transcription factor 1

Isotype

Mouse IgG2a

Contents

243