Anti-TTK/Mps1/TTK Antibody Picoband®

About this product

Boster Bio Anti-TTK/Mps1/TTK Antibody Picoband® catalog # A01132-1. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A01132-1

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Dual specificity protein kinase TTK is a protein that in humans is encoded by the XRCC2 gene. This gene is mapped to 6q14.1. This gene encodes a dual specificity protein kinase with the ability to phosphorylate tyrosine, serine and threonine. Associated with cell proliferation, this protein is essential for chromosome alignment at the centromere during mitosis and is required for centrosome duplication. It has been found to be a critical mitotic checkpoint protein for accurate segregation of chromosomes during mitosis. Tumorigenesis may occur when this protein fails to degrade and produces excess centrosomes resulting in aberrant mitotic spindles. Alternative splicing results in multiple transcript variants.

Synonyms

Dual specificity protein kinase TTK; Phosphotyrosine picked threonine-protein kinase; PYT; TTK; MPS1, MPS1L1

Gene Name

TTK

Gene ID

7272

UniProt

P33981

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human TTK/Mps1/TTK recombinant protein (Position: Q487-S821) .

Clonality

Polyclonal

Tissue Specificity

Present in rapidly proliferating cell lines.

Applications

ELISA, Flow Cytometry, IF, IHC, ICC, WB

Field of Research

Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Phosphorylates proteins on serine, threonine, and tyrosine. Probably associated with cell proliferation. Essential for chromosome alignment by enhancing AURKB activity (via CDCA8 phosphorylation) at the centromere, and for the mitotic checkpoint.

References & Citations

1. Lindberg, R. A., Fischer, W. H., Hunter, T. Characterization of a human protein threonine kinase isolated by screening an expression library with antibodies to phosphotyrosine. Oncogene 8: 351-359, 1993. 2. Mills, G. B., Schmandt, R., McGill, M., Amendola, A., Hill, M., Jacobs, K., May, C., Rodricks, A.-M., Campbell, S., Hogg, D. Expression of TTK, a novel human protein kinase, is associated with cell proliferation. J. Biol. Chem. 267: 16000-16006, 1992. 3. Poss, K. D., Wilson, L. G., Keating, M. T. Heart regeneration in zebrafish. Science 298: 2188-2190, 2002.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Observed Molecular Weight

97 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

TTK protein kinase

Subcellular Location

Spindle. Nucleus. Cytoplasm. Kinetochore. Membrane.

Protein Name

Dual specificity protein kinase TTK

Isotype

Rabbit IgG

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.