Anti-NDRG1 Antibody Picoband®

About this product

Boster Bio Anti-NDRG1 Antibody Picoband® catalog # A01327-1. Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A01327-1

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Protein NDRG1 is a protein that in humans is encoded by the NDRG1 gene. It is a member of the N-myc downregulated gene family which belong to the alpha/beta hydrolase superfamily. The NDRG1 gene is mapped to chromosome 8q24. It has got a deduced 394 amino acid protein with a molecular mass of 43kD. NDRG1 is expressed in the cytoplasm and basolateral membranes of gut lumen surface epithelial cells. The protein appears to play a vital role in growth arrest and cell differentiation.

Synonyms

Protein NDRG1; Differentiation-related gene 1 protein; DRG-1; N-myc downstream-regulated gene 1 protein; Nickel-specific induction protein Cap43; Reducing agents and tunicamycin-responsive protein; RTP; Rit42; NDRG1; CAP43; DRG1; RTP

Gene Name

NDRG1

Gene ID

10397

UniProt

Q92597

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human NDRG1 recombinant protein (Position: S2-N220) .

Clonality

Polyclonal

Tissue Specificity

Ubiquitous; expressed most prominently in placental membranes and prostate, kidney, small intestine, and ovary tissues. Also expressed in heart, brain, skeletal muscle, lung, liver and pancreas. Low levels in peripheral blood leukocytes and in tissues of the immune system. Expressed mainly in epithelial cells. Also found in Schwann cells of peripheral neurons. Reduced expression in adenocarcinomas compared to normal tissues. In colon, prostate and placental membranes, the cells that border the lumen show the highest expression.

Applications

ELISA, Flow Cytometry, IHC, WB

Field of Research

Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Stress-responsive protein involved in hormone responses, cell growth, and differentiation. Acts as a tumor suppressor in many cell types. Necessary but not sufficient for p53/TP53-mediated caspase activation and apoptosis. Has a role in cell trafficking, notably of the Schwann cell, and is necessary for the maintenance and development of the peripheral nerve myelin sheath. Required for vesicular recycling of CDH1 and TF. May also function in lipid trafficking. Protects cells from spindle disruption damage. Functions in p53/TP53-dependent mitotic spindle checkpoint. Regulates microtubule dynamics and maintains euploidy.

References & Citations

1. Hunter, M., Bernard, R., Freitas, E., Boyer, A., Morar, B., Martins, I. J., Tournev, I., Jordanova, A., Guergelcheva, V., Ishpekova, B., Kremensky, I., Nicholson, G., Schlotter, B., Lochmuller, H., Voit, T., Colomer, J., Thomas, P. K., Levy, N., Kalaydjieva, L. Mutation screening of the N-myc downstream-regulated gene 1 (NDRG1) in patients with Charcot-Marie-Tooth disease. Hum. Mutat. 22: 129-135, 2003. 2. Okamoto, Y., Goksungur, M. T., Pehlivan, D., Beck, C. R., Gonzaga-Jauregui, C., Muzny, D. M., Atik, M. M., Carvalho, C. M. B., Matur, Z., Bayraktar, S., Boone, P. M., Akyuz, K., Gibbs, R. A., Battaloglu, E., Parman, Y., Lupski, J. R. Exonic duplication CNV of NDRG1 associated with autosomal-recessive HMSN-Lom/CMT4D. Genet. Med. 16: 386-394, 2014. Note: Erratum: Genet. Med. 16: 203 only, 2014. 3. Okuda, T., Higashi, Y., Kokame, K., Tanaka, C., Kondoh, H., Miyata, T. Ndrg1-deficient mice exhibit a progressive demyelinating disorder of peripheral nerves. Molec. Cell. Biol. 24: 3949-3956, 2004.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

43 kDa

Observed Molecular Weight

46 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

N-myc downstream regulated 1

Subcellular Location

Nucleus. Centrosome. Cytosol. Cell membrane.

Protein Name

Protein NDRG1

Isotype

Rabbit IgG

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.