Anti-PAR1/Thrombin Receptor/F2R Antibody Picoband®

About this product

Boster Bio Anti-PAR1/Thrombin Receptor/F2R Antibody Picoband® catalog # A03352-1. Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A03352-1

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Proteinase-activated receptor 1  (PAR1), also known as the  coagulation factor II (thrombin) receptor,   is a protein  that in humans is encoded by the  F2R  gene. By fluorescence in situ hybridization, this gene is mapped to 5q13, confirming its presence as a single locus in the human genome. PAR1 is a  G protein-coupled receptor  involved in the regulation of  thrombotic  response. Proteolytic cleavage leads to the activation of the receptor. The expression of PAR1 is both required and sufficient to promote growth and invasion of breast carcinoma cells in a xenograft mouse model.&nbsp

Synonyms

Proteinase-activated receptor 1; PAR-1; Coagulation factor II receptor; Thrombin receptor; F2R; CF2R; PAR1; TR

Gene Name

F2R

Gene ID

2149

UniProt

P25116

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human PAR1/Thrombin Receptor/F2R recombinant protein (Position: K130-T425) .

Clonality

Polyclonal

Tissue Specificity

Platelets and vascular endothelial cells.

Applications

ELISA, Flow Cytometry, IHC, WB

Field of Research

Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

High affinity receptor for activated thrombin coupled to G proteins that stimulate phosphoinositide hydrolysis. May play a role in platelets activation and in vascular development.

References & Citations

1. Bahou, W. F., Nierman, W. C., Durkin, A. S., Potter, C. L., Demetrick, D. J. Chromosomal assignment of the human thrombin receptor gene: localization to region q13 of chromosome 5. Blood 82: 1532-1537, 1993. 2. Boire, A., Covic, L., Agarwal, A., Jacques, S., Sherifi, S., Kuliopulos, A. PAR1 is a matrix metalloprotease-1 receptor that promotes invasion and tumorigenesis of breast cancer cells. Cell 120: 303-131, 2005. 3. Chu, H.-W., Cheng, C.-W., Chou, W.-C., Hu, L.-Y., Wang, H.-W., Hsiung, C.-N., Hsu, H.-M., Wu, P.-E., Hou, M.-F., Shen, C.-Y., Yu, J.-C. A novel estrogen receptor-microRNA 190a-PAR-1-pathway regulates breast cancer progression, a finding initially suggested by genome-wide analysis of loci associated with lymph-node metastasis. Hum. Molec. Genet. 23: 355-367, 2014.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Observed Molecular Weight

47 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

Coagulation factor II thrombin receptor

Subcellular Location

Cell membrane. Multi-pass membrane protein.

Protein Name

Proteinase-activated receptor 1

Isotype

Rabbit IgG

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.