Anti-ATP11C Antibody Picoband®

About this product

Boster Bio Anti-ATP11C Antibody Picoband® catalog # A06516-1. Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A06516-1

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

ATP11C is an enzyme that in humans is encoded by the ATP11C gene. This gene is mapped to Xq27.1.

Synonyms

Phospholipid-transporting ATPase IG (EC:7.6.2.1) ; ATPase IQ; ATPase class VI type 11C; P4-ATPase flippase complex alpha subunit ATP11C; ATP11C; ATPIG, ATPIQ

Gene Name

ATP11C

Gene ID

286410

UniProt

Q8NB49

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of human ATP11C, which shares 83.8% and 86.5% amino acid (aa) sequence identity with mouse and rat ATP11C, respectively.

Clonality

Polyclonal

Tissue Specificity

Widely expressed.

Applications

Flow Cytometry, IF, ICC, WB

Field of Research

Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Catalytic component of a P4-ATPase flippase complex which catalyzes the hydrolysis of ATP coupled to the transport of aminophospholipids from the outer to the inner leaflet of various membranes and ensures the maintenance of asymmetric distribution of phospholipids. In the cell membrane of erythrocytes, it is required to maintain phosphatidylserine (PS) in the inner leaflet preventing its exposure on the surface. This asymmetric distribution is critical for the survival of erythrocytes in circulation since externalized PS is a phagocytic signal for splenic macrophages. Phospholipid translocation seems also to be implicated in vesicle formation and in uptake of lipid signaling molecules. Required for B cell differentiation past the pro-B cell stage. Seems to mediate PS flipping in pro-B cells. May be involved in the transport of cholestatic bile acids.

References & Citations

1. Arashiki, N., Takakuwa, Y., Mohandas, N., Hale, J., Yoshida, K., Ogura, H., Utsugisawa, T., Ohga, S., Miyano, S., Ogawa, S., Kojima, S., Kanno, H. ATP11C is a major flippase in human erythrocytes and its defect causes congenital hemolytic anemia. Haematologica 101: 559-565, 2016. 2. Nesbit, M. A., Bowl, M. R., Harding, B., Schlessinger, D., Whyte, M. P., Thakker, R. V. X-linked hypoparathyroidism region on Xq27 is evolutionarily conserved with regions on 3q26 and 13q34 and contains a novel P-type ATPase. Genomics 84: 1060-1070, 2004. 3. Segawa, K., Kurata, S., Yanagihashi, Y., Brummelkamp, T. R., Matsuda, F., Nagata, S. Caspase-mediated cleavage of phospholipid flippase for apoptotic phosphatidylserine exposure. Science 344: 1164-1168, 2014.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

27687 MW

Observed Molecular Weight

129 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

ATPase phospholipid transporting 11C

Subcellular Location

Endoplasmic reticulum membrane. Cell membrane. Multi-pass membrane protein.

Protein Name

Phospholipid-transporting ATPase IG

Isotype

Rabbit IgG

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.