Anti-SYP/Synaptophysin Antibody Picoband® (monoclonal, 3G12)

About this product

Boster Bio Anti-SYP/Synaptophysin Antibody Picoband® (monoclonal, 3G12) catalog # M05049-3. Tested in WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

M05049-3

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

SYP (Synaptophysin) is located on the short arm of X chromosome (Xp11.23-p11.22) . The protein is a synaptic vesicle glycoprotein with four transmembrane domains weighing 38kDa. It is present inneuroendocrine cells and in virtually all neurons in the brain and spinal cord that participate in synaptic transmission. It acts as a marker for neuroendocrine tumors, and its ubiquity at the synapse has led to the use of synaptophysin immunostaining for quantification of synapses. Also, the protein binds cholesterol and is thought to targeting of vesicle-associated membrane protein 2 (synaptobrevin) to intracellular compartments. Mutations in this gene are associated with X-linked mental retardation (XLMR) .

Synonyms

MRXSYP|Major synaptic vesicle protein p38

Gene Name

SYP

Gene ID

6855

UniProt

Human (P08247), Mouse (Q62277), Rat (P07825)

Host

Mouse

Reactivity

Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human Synaptophysin, identical to the related mouse and rat sequences.

Clonality

Monoclonal

Clone

Clone: 3G12

Tissue Specificity

Isoform CFL2b is expressed predominantly in skeletal muscle and heart. Isoform CFL2a is expressed in various tissues.

Applications

WB

Field of Research

Cell Adhesion Proteins, Epigenetics and Nuclear Signaling, Integrins, Mediator Complex, Neuroscience, Receptors, Transcription

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500μg/ml.

Function

Possibly involved in structural functions as organizing other membrane components or in targeting the vesicles to the plasma membrane.Involved in the regulation of short-term and long-term synaptic plasticity (By similarity) .

References & Citations

1. Calhoun ME, Jucker M, Martin LJ, Thinakaran G, Price DL, Mouton PR (December 1996) . Comparative evaluation of synaptophysin-based methods for quantification of synapses. J. Neurocytol.25 (12) : 821–8.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Observed Molecular Weight

38 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

Western blot, 0.1-0.5μg/ml, Mouse, Rat

Gene Name Synonym

Synaptophysin

Subcellular Location

Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane; Multi-pass membrane protein. Cell junction, synapse, synaptosome.

Protein Name

Synaptophysin

Isotype

Mouse IgG2b

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.