Anti-HMG4 Antibody Picoband® (monoclonal, 8H9)

About this product

Boster Bio Anti-HMG4 Antibody Picoband® (monoclonal, 8H9) catalog # M02834-1. Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

M02834-1

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

High-mobility group protein B, also known as HMG4, is a protein that in humans is encoded by the HMGB3 gene. This gene encodes a member of a family of proteins containing one or more high mobility group DNA-binding motifs. The encoded protein plays an important role in maintaining stem cell populations, and may be aberrantly expressed in tumor cells. A mutation in this gene was associated with microphthalmia, syndromic 13. There are numerous pseudogenes of this gene on multiple chromosomes. Alternative splicing results in multiple transcript variants.

Synonyms

Chromosomal protein, Nonhistone, HMG4 antibody|High mobility group (nonhistone chromosomal) protein 4 antibody|High mobility group box 3 antibody|High mobility group protein 2a antibody|High mobility group protein 4 antibody|High mobility group protein B3 antibody| High mobility group protein HMG4 antibody|HMG 4 antibody|HMG-2a antibody|HMG-4 antibody|HMG2A antibody|HMGB 3 antibody|HMGB3 antibody| HMGB3_HUMAN antibody|MGC90319 antibody|Non histone chromosomal protein antibody|Nonhistone chromosomal protein HMG4 antibody

Gene Name

HMGB3

Gene ID

3149

UniProt

O15347

Host

Mouse

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human HMG4, identical to the related mouse and rat sequences.

Clonality

Monoclonal

Clone

Clone: 8H9

Tissue Specificity

Expressed predominantly in placenta.

Applications

Flow Cytometry, IF, ICC, WB

Field of Research

Cell Biology, Protease Inhibitors, Proteolysis/Ubiquitin

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500μg/ml.

Function

Multifunctional protein with various roles in different cellular compartments, May act in a redox sensitive manner.Associates with chromatin and binds DNA with a preference to non- canonical DNA structures such as single-stranded DNA. Can bent DNA and enhance DNA flexibility by looping thus providing a mechanism to promote activities on various gene promoters (By similarity) .Proposed to be involved in the innate immune response to nucleic acids by acting as a cytoplasmic promiscuous immunogenic DNA/RNA sensor (By similarity) .Negatively regulates B-cell and myeloid cell differentiation.In hematopoietic stem cells may regulate the balance between self-renewal and differentiation. Involved in negative regulation of canonical Wnt signaling (By similarity) .

References & Citations

1. Entrez Gene: HMGB3 high-mobility group box 3. 2. Davis DL, Burch JB (1992) . Isolation of a chicken HMG2 cDNA clone and evidence for an HMG2-specific 3'-untranslated region.. Gene 113 (2) : 251–6. 3. Vaccari T, Beltrame M, Ferrari S, Bianchi ME (Aug 1998) . Hmg4, a new member of the Hmg1/2 gene family.Genomics 49 (2) : 247–52.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Observed Molecular Weight

23 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

High mobility group box 3

Subcellular Location

Nucleus . Chromosome . Cytoplasm .

Protein Name

High mobility group protein B3

Isotype

Mouse IgG2b

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.