Anti-CBR1 Picoband® Antibody

About this product

Boster Bio Anti-CBR1 Picoband® Antibody catalog # A02825-1. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A02825-1

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Carbonyl reductase 1, also known as CBR1, is an enzyme which in humans is encoded by the CBR1 gene. It is mapped to 21q22.12. The protein encoded by this gene belongs to the short-chain dehydrogenases/reductases (SDR) family, which function as NADPH-dependent oxidoreductases having wide specificity for carbonyl compounds, such as quinones, prostaglandins, and various xenobiotics. Alternatively spliced transcript variants have been found for this gene.

Synonyms

Carbonyl reductase [NADPH] 1; 15-hydroxyprostaglandin dehydrogenase [NADP (+) ]; NADPH-dependent carbonyl reductase 1; Prostaglandin 9-ketoreductase; Prostaglandin-E (2) 9-reductase; Short chain dehydrogenase/reductase family 21C member 1; CBR1; CBR; CRN; SDR21C1

Gene Name

CBR1

Gene ID

873

UniProt

P16152

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human CBR1 recombinant protein (Position: N14-E262) .

Clonality

Polyclonal

Tissue Specificity

Highly expressed in spleen. Also detected in liver, placenta, lung, peripheral blood leukocytes and in brain.

Applications

ELISA, Flow Cytometry, IF, IHC, ICC, WB

Field of Research

Cell Biology, Co-Activators/Co-Repressors, Epigenetics and Nuclear Signaling, Nuclear Receptors, Nuclear Signaling Pathways, Proteasome / Ubiquitin, Proteolysis/Ubiquitin, Transcription, Transcription Factors, Ubiquitin & Ubiquitin Like Modifiers

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

NADPH-dependent reductase with broad substrate specificity. Catalyzes the reduction of a wide variety of carbonyl compounds including quinones, prostaglandins, menadione, plus various xenobiotics. Catalyzes the reduction of the antitumor anthracyclines doxorubicin and daunorubicin to the cardiotoxic compounds doxorubicinol and daunorubicinol. Can convert prostaglandin E2 to prostaglandin F2-alpha. Can bind glutathione, which explains its higher affinity for glutathione-conjugated substrates. Catalyzes the reduction of S-nitrosoglutathione.

References & Citations

1. Avramopoulos, D., Cox, T., Forrest, G. L., Chakravarti, A., Antonarakis, S. E. Linkage mapping of the carbonyl reductase (CBR) gene on human chromosome 21 using a DNA polymorphism in the 3-prime untranslated region. Genomics 13: 447-448, 1992. 2. Forrest, G. L., Akman, S., Krutzik, S., Paxton, R. J., Sparkes, R. S., Doroshow, J., Felsted, R. L., Glover, C. J., Mohandas, T., Bachur, N. R. Induction of a human carbonyl reductase gene located on chromosome 21. Biochim. Biophys. Acta 1048: 149-155, 1990. 3. Lemieux, N., Malfoy, B., Forrest, G. L. Human carbonyl reductase (CBR) localized to band 21q22.1 by high-resolution fluorescence in situ hybridization displays gene dosage effects in trisomy 21 cells. Genomics 15: 169-172, 1993.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

96950 MW

Observed Molecular Weight

36 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

Carbonyl reductase 1

Subcellular Location

Cytoplasm.

Protein Name

Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.