Anti-MAFA Picoband® Antibody

About this product

Boster Bio Anti-MAFA Picoband® Antibody catalog # A05251-1. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A05251-1

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

MAFA is a transcription factor that binds RIPE3b, a conserved enhancer element that regulates pancreatic beta cell-specific expression of the insulin gene. It is mapped to 8q24.3.

Synonyms

Transcription factor MafA; Pancreatic beta-cell-specific transcriptional activator; RIPE3b1 factor; V-maf musculoaponeurotic fibrosarcoma oncogene homolog A; MAFA

Gene Name

MAFA

Gene ID

389692

UniProt

Q8NHW3

Host

Rabbit

Reactivity

Human, Mouse

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human MAFA recombinant protein (Position: A9-D308) .

Clonality

Polyclonal

Tissue Specificity

Preferentially expressed in regulatory T-cells (Tregs) .

Applications

ELISA, Flow Cytometry, IF, IHC, ICC, WB

Field of Research

Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Transcription factor that activates insulin gene expression. Acts synergistically with NEUROD1/BETA2 and PDX1. Binds the insulin enhancer C1/RIPE3b element. Binds to consensus TRE-type MARE 5'-TGCTGACTCAGCA-3' DNA sequence.

References & Citations

1. Iacovazzo, D., Flanagan, S. E., Walker, E., Quezado, R., de Sousa Barros, F. A., Caswell, R., Johnson, M. B., Wakeling, M., Brandle, M., Guo, M., Dang, M. N., Gabrovska, P., and 14 others. MAFA missense mutation causes familial insulinomatosis and diabetes mellitus. Proc. Nat. Acad. Sci. 115: 1027-1032, 2018. 2. Olbrot, M., Rud, J., Moss, L. G., Sharma, A. Identification of beta-cell-specific insulin gene transcription factor RIPE3b1 as mammalian MafA. Proc. Nat. Acad. Sci. 99: 6737-6742, 2002. 3. Tragl, K.-H., Mayr, W. R. Familial islet-cell adenomatosis. Lancet 318: 426-428, 1977. Note: Originally Volume II.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

39411 MW

Observed Molecular Weight

50 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

MAF bZIP transcription factor A

Subcellular Location

Nucleus.

Protein Name

Zinc finger protein Helios

Isotype

Rabbit IgG

Contents

243