- Catalog number
- A02430-1
- Size / count
- 100 µg/Vial
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- The PHLPP isoforms (PH domain and Leucine rich repeat Protein Phosphatases) are a pair of protein phosphatases, PHLPP1 and PHLPP2, that are important regulators of Akt serine-threonine kinases (Akt1, Akt2, Akt3) and conventional/novel protein kinase C (PKC) isoforms. It is mapped to 18q21.33. This gene encodes a member of the serine/threonine phosphatase family. The encoded protein promotes apoptosis by dephosphorylating and inactivating the serine/threonine kinase Akt, and functions as a tumor suppressor in multiple types of cancer. Increased expression of this gene may also play a role in obesity and type 2 diabetes by interfering with Akt-mediated insulin signaling.
- Synonyms
- PH domain leucine-rich repeat-containing protein phosphatase 1; Pleckstrin homology domain-containing family E member 1; PH domain-containing family E member 1; Suprachiasmatic nucleus circadian oscillatory protein; hSCOP; PHLPP1; KIAA0606; PHLPP; PLEKHE1; SCOP
- Gene Name
- PHLPP1
- Gene ID
- 23239
- UniProt
- O60346
- Host
- Rabbit
- Reactivity
- Human
- Cross Reactivity
- No cross-reactivity with other proteins.
- Immunogen
- E.coli-derived human SCOP/PHLPP1 recombinant protein (Position: R1083-A1436) .
- Clonality
- Polyclonal
- Tissue Specificity
- Monocyte/macrophage specific.
- Applications
- WB, ELISA
- Field of Research
- Immunoglobulins, Immunology, Innate Immunity
- Purification
- Immunogen affinity purified.
- Concentration
- Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
- Form
- Lyophilized
- Reconstitution
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
- Function
- Protein phosphatase involved in regulation of Akt and PKC signaling. Mediates dephosphorylation in the C-terminal domain hydrophobic motif of members of the AGC Ser/Thr protein kinase family; specifically acts on 'Ser-473' of AKT2 and AKT3, 'Ser-660' of PRKCB and 'Ser-657' of PRKCA. Isoform 2 seems to have a major role in regulating Akt signaling in hippocampal neurons. Akt regulates the balance between cell survival and apoptosis through a cascade that primarily alters the function of transcription factors that regulate pro- and antiapoptotic genes. Dephosphorylation of 'Ser-473' of Akt triggers apoptosis and suppression of tumor growth. Dephosphorylation of PRKCA and PRKCB leads to their destabilization and degradation. Dephosphorylates STK4 on 'Thr-387' leading to STK4 activation and apoptosis. Dephosphorylates RPS6KB1 and is involved in regulation of cap-dependent translation. Inhibits cancer cell proliferation and may act as a tumor suppressor. Dephosphorylates RAF1 inhibiting its kinase activity. May act as a negative regulator of K-Ras signaling in membrane rafts. Involved in the hippocampus-dependent long-term memory formation. Involved in circadian control by regulating the consolidation of circadian periodicity after resetting. Involved in development and function of regulatory T-cells.
- References & Citations
- 1. Brognard, J., Sierecki, E., Gao, T., Newton, A. C. PHLPP and a second isoform, PHLPp2, differentially attenuate the amplitude of Akt signaling by regulating distinct Akt isoforms. Molec. Cell 25: 917-931, 2007. 2. Chen, H. H., Handel, N., Ngeow, J., Muller, J., Huhn, M., Yang, H.-T., Heindl, M., Berbers, R.-M., Hegazy, A. N., Kionke, J., Yehia, L., Sack, U., and 15 others. Immune dysregulation in patients with PTEN hamartoma tumor syndrome.: analysis of FOXP3 regulatory T cells. J. Allergy Clin. Immun. 139: 607-620, 2017. 3. Cheng, H.-Y. M., Papp, J. W., Varlamova, O., Dziema, H., Russell, B., Curfman, J. P., Nakazawa, T., Shimizu, K., Okamura, H., Impey, S., Obrietan, K. microRNA modulation of circadian-clock period and entrainment. Neuron 54: 813-829, 2007.
- Storage Conditions
- Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
- Calculated Molecular Weight
- 38779 MW
- Observed Molecular Weight
- 140 kDa
- Specificity
- No cross reactivity with other proteins.
- Gene Name Synonym
- PH domain and leucine rich repeat protein phosphatase 1
- Subcellular Location
- Nucleus. Cytoplasm. Membrane. Peripheral membrane protein. Cell membrane.
- Protein Name
- BAG family molecular chaperone regulator 1
- Isotype
- Rabbit IgG
- Contents
- 243
