Anti-PPM1A Picoband® Antibody

About this product

Boster Bio Anti-PPM1A Picoband® Antibody catalog # A02928-1. Tested in ELISA, ICC/IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A02928-1

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Protein phosphatase 1A is an enzyme that in humans is encoded by the PPM1A gene. It is mapped to 14q23.1. The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase dephosphorylates, and negatively regulates the activities of, MAP kinases and MAP kinase kinases. It has been shown to inhibit the activation of p38 and JNK kinase cascades induced by environmental stresses. This phosphatase can also dephosphorylate cyclin-dependent kinases, and thus may be involved in cell cycle control. Overexpression of this phosphatase is reported to activate the expression of the tumor suppressor gene TP53/p53, which leads to G2/M cell cycle arrest and apoptosis. Three alternatively spliced transcript variants encoding distinct isoforms have been described.

Synonyms

Protein phosphatase 1A; Protein phosphatase 2C isoform alpha; PP2C-alpha; Protein phosphatase IA; PPM1A; PPPM1A

Gene Name

PPM1A

Gene ID

5494

UniProt

P35813

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human PPM1A, identical to the related mouse and rat sequences.

Clonality

Polyclonal

Tissue Specificity

Ubiquitous with higher expression in heart, brain and skeletal muscle.

Applications

ELISA, ICC/IF, IHC, WB

Field of Research

Cancer, Cardiovascular, Fatty Acid Oxidation, Fatty Acids, Lipid and Lipoprotein Metabolism, Lipid Metabolism, Lipids/Lipoproteins, Metabolic Signaling Pathways, Metabolism, Pathways and Processes, Redox Metabolism, Signal Transduction, Tumor Biomarkers

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Enzyme with a broad specificity. Negatively regulates TGF-beta signaling through dephosphorylating SMAD2 and SMAD3, resulting in their dissociation from SMAD4, nuclear export of the SMADs and termination of the TGF-beta-mediated signaling. Dephosphorylates PRKAA1 and PRKAA2. Plays an important role in the termination of TNF-alpha-mediated NF-kappa-B activation through dephosphorylating and inactivating IKBKB/IKKB.

References & Citations

1. Lin, X., Duan, X., Liang, Y.-Y., Su, Y., Wrighton, K. H., Long, J., Hu, M., Davis, C. M., Wang, J., Brunicardi, F. C., Shi, Y., Chen, Y.-G., Meng, A., Feng, X.-H. PPM1A functions as a Smad phosphatase to terminate TGF-beta signaling. Cell 125: 915-928, 2006. Note: Editorial Expression of Concern: Cell 165: 498 only, 2016. Editoral Note: Cell 166: 1597 only, 2016. 2. Mann, D. J., Campbell, D. G., McGowan, C. H., Cohen, P. T. W. Mammalian protein serine/threonine phosphatase 2C: cDNA cloning and comparative analysis of amino acid sequences. Biochim. Biophys. Acta 1130: 100-104, 1992. 3. Takekawa, M., Maeda, T., Saito, H. Protein phosphatase 2C-alpha inhibits the human stress-responsive p38 and JNK MAPK pathways. EMBO J. 17: 4744-4752, 1998.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

42 kDa

Observed Molecular Weight

40 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Protein phosphatase, Mg2+/Mn2+ dependent 1A

Subcellular Location

Nucleus. Cytosol. Membrane. Lipid-anchor.

Protein Name

Peroxisomal multifunctional enzyme type 2

Isotype

Rabbit IgG

Contents

243