Anti-COX2/Cyclooxygenase 2/PTGS2 Picoband® Antibody

About this product

Boster Bio Anti-COX2/Cyclooxygenase 2/PTGS2 Picoband® Antibody catalog # A00084-2. Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A00084-2

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Prostaglandin-endoperoxide synthase 2, also known as cyclooxygenase-2 or COX-2, is an enzyme that in humans is encoded by the PTGS2 gene. It is mapped to 1q31.1. Prostaglandin-endoperoxide synthase (PTGS), also known as cyclooxygenase, is the key enzyme in prostaglandin biosynthesis, and acts both as a dioxygenase and as a peroxidase. There are two isozymes of PTGS: a constitutive PTGS1 and an inducible PTGS2, which differ in their regulation of expression and tissue distribution. This gene encodes the inducible isozyme. It is regulated by specific stimulatory events, suggesting that it is responsible for the prostanoid biosynthesis involved in inflammation and mitogenesis.

Synonyms

Prostaglandin G/H synthase 2; Cyclooxygenase-2; COX-2; PHS II; Prostaglandin H2 synthase 2; PGH synthase 2; PGHS-2; Prostaglandin-endoperoxide synthase 2; PTGS2; COX2

Gene Name

PTGS2

Gene ID

5743

UniProt

P35354

Host

Rabbit

Reactivity

Human, Mouse

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human COX2/Cyclooxygenase 2/PTGS2 recombinant protein (Position: A18-L604) .

Clonality

Polyclonal

Tissue Specificity

Expressed in testis. Detected in samples of kidney, brain and skin.

Applications

ELISA, Flow Cytometry, IHC, WB

Field of Research

Atherosclerosis, Cancer, Cancer Metabolism, Cardiovascular, Cholesterol Metabolism, Endocrine Metabolism, Heart Disease, Hormone Biosynthesis, Lipid and Lipoprotein Metabolism, Lipid Metabolism, Lipid Signaling, Lipids/Lipoproteins, Metabolic Signaling Pathway, Metabolic Signaling Pathways, Metabolism, Pathways and Processes, Platelets, Signal Transduction, Signaling Pathway, Tumor Biomarkers

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Converts arachidonate to prostaglandin H2 (PGH2), a committed step in prostanoid synthesis. Constitutively expressed in some tissues in physiological conditions, such as the endothelium, kidney and brain, and in pathological conditions, such as in cancer. PTGS2 is responsible for production of inflammatory prostaglandins. Up-regulation of PTGS2 is also associated with increased cell adhesion, phenotypic changes, resistance to apoptosis and tumor angiogenesis. In cancer cells, PTGS2 is a key step in the production of prostaglandin E2 (PGE2), which plays important roles in modulating motility, proliferation and resistance to apoptosis. During neuroinflammation, plays a role in neuronal secretion of specialized preresolving mediators (SPMs), especially 15-R-lipoxin A4, that regulates phagocytic microglia.

References & Citations

1. Kosaka, T., Miyata, A., Ihara, H., Hara, S., Sugimoto, T., Takeda, O., Takahashi, E., Tanabe, T. Characterization of the human gene (PTGS2) encoding prostaglandin-endoperoxide synthase 2. Europ. J. Biochem. 221: 889-897, 1994. 2. Tay, A., Squire, J. A., Goldberg, H., Skorecki, K. Assignment of the human prostaglandin-endoperoxide synthase 2 (PTGS2) gene to 1q25 by fluorescence in situ hybridization. Genomics 23: 718-719, 1994. 3. Xu, L., Zhang, L., Yi, Y., Kang, H.-K., Datta, S. K. Human lupus T cells resist inactivation and escape death by upregulating COX-2. Nature Med. 10: 411-415, 2004.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

68996 MW

Observed Molecular Weight

75 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

Prostaglandin-endoperoxide synthase 2

Subcellular Location

Microsome membrane. Peripheral membrane protein. Endoplasmic reticulum membrane. Peripheral membrane protein.

Protein Name

Prostaglandin G/H synthase 2

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg NaN3.