Anti-alpha 1 Catenin/CTNNA1 Antibody Picoband® (monoclonal, 10I2)

About this product

Boster Bio Anti-alpha 1 Catenin/CTNNA1 Antibody Picoband® (monoclonal, 10I2) catalog # M01617-1. Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

M01617-1

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

CTNNA1, also known as Catenin alpha-1 or Catenin (cadherin-associated protein), alpha 1, is a protein that in humans is encoded by the CTNNA1 gene. It is mapped to 5q31.2. When surface epithelium CTNNA1 was ablated, hair follicle development was blocked and epidermal morphogenesis was dramatically affected, with defects in adherens junction formation, intercellular adhesion, and epithelial polarity. In vitro, CTNNA1 null keratinocytes were poorly contact inhibited and grew rapidly. These differences were not dependent upon intercellular adhesion and were in marked contrast to keratinocytes conditionally null for another essential intercellular adhesion protein, desmoplakin Knockout keratinocytes exhibited sustained activation of the Ras-MAPK cascade due to aberrations in growth factor responses. It is concluded that features of precancerous lesions often attributed to defects in cell cycle regulatory genes can be generated by compromising the function of CTNNA1.

Synonyms

Alpha catenin antibody; alpha E catenin antibody; Alpha E-catenin antibody; alphaE catenin antibody; Cadherin associated protein 102kDa antibody; Cadherin associated protein antibody; Cadherin-associated protein antibody; CAP 102 antibody; CAP102 antibody; Catenin (cadherin associated protein) alpha 1 102kDa antibody; Catenin (cadherin associated protein), alpha 1, 102kDa antibody; Catenin alpha 1 antibody; Catenin alpha-1 antibody; CTNA1_HUMAN antibody; CTNNA 1 antibody; CTNNA1 antibody; FLJ36832 antibody; FLJ52416 antibody; NY REN 13 antigen antibody; OTTHUMP00000224141 antibody; OTTHUMP00000224147 antibody; Renal carcinoma antigen NY REN 13 antibody; Renal carcinoma antigen NY-REN-13 antibody

Gene Name

CTNNA1

Gene ID

1495

UniProt

P35221

Host

Mouse

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human CTNNA1 recombinant protein (Position: D143-D292) . Human CTNNA1 shares 98% amino acid (aa) sequence identity with mouse CTNNA1.

Clonality

Monoclonal

Clone

Clone: 10I2

Tissue Specificity

Expressed ubiquitously in normal tissues.

Applications

Flow Cytometry, IHC, WB

Field of Research

Actin Binding Proteins, Actin, etc., Cytoskeleton, Cytoskeleton/ECM, Microfilaments, Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Associates with the cytoplasmic domain of a variety of cadherins. The association of catenins to cadherins produces a complex which is linked to the actin filament network, and which seems to be of primary importance for cadherins cell-adhesion properties. Can associate with both E- and N-cadherins. Originally believed to be a stable component of E-cadherin/catenin adhesion complexes and to mediate the linkage of cadherins to the actin cytoskeleton at adherens junctions. In contrast, cortical actin was found to be much more dynamic than E-cadherin/catenin complexes and CTNNA1 was shown not to bind to F-actin when assembled in the complex suggesting a different linkage between actin and adherens junctions components. The homodimeric form may regulate actin filament assembly and inhibit actin branching by competing with the Arp2/3 complex for binding to actin filaments. May play a crucial role in cell differentiation.

References & Citations

1. Vasioukhin, V., Bauer, C., Degenstein, L., Wise, B., Fuchs, E. Hyperproliferation and defects in epithelial polarity upon conditional ablation of alpha-catenin in skin. Cell 104: 605-617, 2001. 2. Herrenknecht K, Ozawa M, Eckerskorn C, Lottspeich F, Lenter M, Kemler R (November 1991) . The uvomorulin-anchorage protein alpha catenin is a vinculin homologue. Proc Natl Acad Sci U S A 88 (20) : 9156–60. 3. Entrez Gene: CTNNA1 catenin (cadherin-associated protein), alpha 1, 102kDa

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Observed Molecular Weight

100 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

Catenin (cadherin-associated protein), alpha 1, 102kDa

Subcellular Location

Cytoskeleton. Cell membrane. Peripheral membrane protein. Cytoplasmic side. Adherens junction. Cell junction.

Protein Name

Activating transcription factor 1

Isotype

Mouse IgG1

Contents

243