Anti-SMAD5 Antibody Picoband®

About this product

Boster Bio Anti-SMAD5 Antibody Picoband® catalog # A01423. Tested in WB applications. This antibody reacts with Human, Monkey, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A01423

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Mothers against decapentaplegic homolog 5 also known as SMAD5 is a protein that in humans is encoded by the SMAD5 gene. It is mapped to 5q31.1. The protein encoded by this gene is involved in the transforming growth factor beta signaling pathway that results in an inhibition of the proliferation of hematopoietic progenitor cells. The encoded protein is activated by bone morphogenetic proteins type 1 receptor kinase, and may be involved in cancer. Alternative splicing results in multiple transcript variants.

Synonyms

Mothers against decapentaplegic homolog 5; MAD homolog 5; Mothers against DPP homolog 5; JV5-1; SMAD family member 5; SMAD 5; Smad5; hSmad5; SMAD5; MADH5

Gene Name

SMAD5

Gene ID

4090

UniProt

Q99717

Host

Rabbit

Reactivity

Human, Monkey, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of human SMAD5, which shares 95.5% amino acid (aa) sequence identity with both mouse and rat SMAD5.

Clonality

Polyclonal

Tissue Specificity

Ubiquitous.

Applications

WB

Field of Research

Class I, Cytoskeleton, Cytoskeleton/ECM, Intermediate Filaments, Keratin, Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD5 is a receptor-regulated SMAD (R-SMAD) .

References & Citations

1. Bruno, E., Horrigan, S. K., Van Den Berg, D., Rozler, E., Fitting, P. R., Moss, S. T., Westbrook, C., Hoffman, R. The Smad5 gene is involved in the intracellular signaling pathways that mediate the inhibitory effects of transforming growth factor-beta on human hematopoiesis. Blood 91: 1917-1923, 1998. 2. Davis, B. N., Hilyard, A. C., Lagna, G., Hata, A. SMAD proteins control DROSHA-mediated microRNA maturation. Nature 454: 56-61, 2008. 3. Gemma, A., Hagiwara, K., Vincent, F., Ke, Y., Hancock, A. R., Nagashima, M., Bennett, W. P., Harris, C. C. hSmad5 gene, a human hSmad family member: its full length cDNA, genomic structure, promoter region and mutation analysis in human tumors. Oncogene 16: 951-956, 1998.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

53704 MW

Observed Molecular Weight

52 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

SMAD family member 5

Subcellular Location

Nucleus. Cytoplasm.

Protein Name

Keratin, type II cytoskeletal 8

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.