Anti-Cyclin E1/CCNE1 Antibody Picoband®

About this product

Boster Bio Anti-Cyclin E1/CCNE1 Antibody Picoband® catalog # A00543-2. Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A00543-2

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

G1/S-specific cyclin-E1 is a protein that in humans is encoded by the CCNE1 gene. It is mapped to 19q12. The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition. This protein accumulates at the G1-S phase boundary and is degraded as cells progress through S phase. Overexpression of this gene has been observed in many tumors, which results in chromosome instability, and thus may contribute to tumorigenesis. This protein was found to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a critical role in promoting cell-cycle progression in the absence of pRB.

Synonyms

G1/S-specific cyclin-E1; CCNE1; CCNE

Gene Name

CCNE1

Gene ID

898

UniProt

P24864

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human Cyclin E1/CCNE1 recombinant protein (Position: R3-A386) .

Clonality

Polyclonal

Tissue Specificity

Highly expressed in testis and placenta. Low levels in bronchial epithelial cells.

Applications

WB, IHC, Flow Cytometry, ELISA

Field of Research

Angiogenesis, Cancer, Cancer Metabolism, Cardiovascular, Growth Factors, Invasion/Microenvironment, Metabolism, Metabolism Processes, Pathways and Processes, Protein Phosphorylation, Response To Hypoxia, Signal Transduction, Tyrosine Kinases

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Essential for the control of the cell cycle at the G1/S (start) transition.

References & Citations

1. Ashworth, L. K., Batzer, M. A., Brandriff, B., Branscomb, E., de Jong, P., Garcia, E., Garnes, J. A., Gordon, L. A., Lamerdin, J. E., Lennon, G., Mohrenweiser, H., Olsen, A. S., Slezak, T., Carrano, A. V. An integrated metric physical map of human chromosome 19. Nature Genet. 11: 422-427, 1995. 2. Demetrick, D. J., Matsumoto, S., Hannon, G. J., Okamoto, K., Xiong, Y., Zhang, H., Beach, D. H. Chromosomal mapping of the genes for the human cell cycle proteins cyclin C (CCNC), cyclin E (CCNE), p21 (CDKN1) and KAP (CDKN3) . Cytogenet. Cell Genet. 69: 190-192, 1995. 3. Geng, Y., Whoriskey, W., Park, M. Y., Bronson, R. T., Medema, R. H., Li, T., Weinberg, R. A., Sicinski, P. Rescue of cyclin D1 deficiency by knockin cyclin E. Cell 97: 767-777, 1999.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Observed Molecular Weight

47 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

Cyclin E1

Subcellular Location

Nucleus.

Isotype

Rabbit IgG

Contents

243