Anti-EAPP Antibody Picoband®

About this product

Boster Bio Anti-EAPP Antibody Picoband® catalog # A07290-1. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A07290-1

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

E2F-associated phosphoprotein is a protein that in humans is encoded by the EAPP gene. It is mapped to 14q13.1. This gene encodes a phosphoprotein that interacts with several members of the E2F family of proteins. The protein localizes to the nucleus, and is present throughout the cell cycle except during mitosis. It functions to modulate E2F-regulated transcription and stimulate proliferation. Alternative splicing results in multiple transcript variants.

Synonyms

E2F-associated phosphoprotein; EAPP; C14orf11; BM-036

Gene Name

EAPP

Gene ID

55837

UniProt

Q56P03

Host

Rabbit

Reactivity

Human, Mouse

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human EAPP recombinant protein (Position: M1-S285) .

Clonality

Polyclonal

Tissue Specificity

Ubiquitously expressed. Highest levels in heart, placenta, skeletal muscle and pancreas. Lower levels in brain, lung and kidney. In the brain, expressed in all regions with high levels in the cerebellum and cerebral cortex. Expressed in COS1 and transformed skin fibroblasts.

Applications

WB, IHC, ICC, IF, Flow Cytometry, ELISA

Field of Research

Interleukins

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

May play an important role in the fine-tuning of both major E2F1 activities, the regulation of the cell-cycle and the induction of apoptosis. Promotes S-phase entry, and inhibits p14 (ARP) expression.

References & Citations

1. Kamnasaran, D., Chen, C.-P., Devriendt, K., Mehta, L., Cox, D. W. Defining a holoprosencephaly locus on human chromosome 14q13 and characterization of potential candidate genes. Genomics 85: 608-621, 2005. 2. Novy, M., Pohn, R., Andorfer, P., Novy-Weiland, T., Galos, B., Schwarzmayr, L., Rotheneder, H. EAPP, a novel E2F binding protein that modulates E2F-dependent transcription. Molec. Biol. Cell 16: 2181-2190, 2005.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

67166 MW

Observed Molecular Weight

45 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

E2F associated phosphoprotein

Subcellular Location

Nucleus. Cytoplasm.

Protein Name

Suppressor of tumorigenicity 7 protein

Isotype

Rabbit IgG

Contents

243