Anti-EPS15 Antibody Picoband®

About this product

Boster Bio Anti-EPS15 Antibody Picoband® catalog # A01681-1. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A01681-1

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Epidermal growth factor receptor substrate 15 is a protein that in humans is encoded by the EPS15 gene. It is mapped to 1p32.3. This gene encodes a protein that is part of the EGFR pathway. The protein is present at clatherin-coated pits and is involved in receptor-mediated endocytosis of EGF. Notably, this gene is rearranged with the HRX/ALL/MLL gene in acute myelogeneous leukemias. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

Synonyms

Epidermal growth factor receptor substrate 15; Protein Eps15; Protein AF-1p; EPS15; AF1P

Gene Name

EPS15

Gene ID

2060

UniProt

P42566

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human EPS15 recombinant protein (Position: M1-K295) .

Clonality

Polyclonal

Tissue Specificity

Ubiquitously expressed.

Applications

WB, Flow Cytometry, ELISA

Field of Research

Adapters, Signal Transduction, Transmembrane

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Involved in cell growth regulation. May be involved in the regulation of mitogenic signals and control of cell proliferation. Involved in the internalization of ligand-inducible receptors of the receptor tyrosine kinase (RTK) type, in particular EGFR. Plays a role in the assembly of clathrin-coated pits (CCPs) . Acts as a clathrin adapter required for post-Golgi trafficking. Seems to be involved in CCPs maturation including invagination or budding. Involved in endocytosis of integrin beta-1 (ITGB1) and transferrin receptor (TFR) ; internalization of ITGB1 as DAB2-dependent cargo but not TFR seems to require association with DAB2.

References & Citations

1. Bakowska, J. C., Jenkins, R., Pendleton, J., Blackstone, C. The Troyer syndrome (SPG20) protein spartin interacts with Eps15. Biochem. Biophys. Res. Commun. 334: 1042-1048, 2005. 2. Bernard, O. A., Mauchauffe, M., Mecucci, C., Van Den Berghe, H., Berger, R. A novel gene, AF-1p, fused to HRX in t (1;11) (p32; q23), is not related to AF-4, AF-9 nor ENL. Oncogene 9: 1039-1045, 1994. 3. Coda, L., Salcini, A. E., Confalonieri, S., Pelicci, G., Sorkina, T., Sorkin, A., Pelicci, P. G., Di Fiore, P. P. Eps15R is a tyrosine kinase substrate with characteristics of a docking protein possibly involved in coated pits-mediated internalization. J. Biol. Chem. 273: 3003-3012, 1998.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

50514 MW

Observed Molecular Weight

138 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

Epidermal growth factor receptor pathway substrate 15

Subcellular Location

Cell membrane. Peripheral membrane protein. Cytoplasmic side. Cytoplasm. Clathrin-coated pit.

Protein Name

Carboxypeptidase M

Isotype

Rabbit IgG

Contents

243