Anti-Lin28/LIN28A Antibody Picoband®

About this product

Boster Bio Anti-Lin28/LIN28A Antibody Picoband® catalog # A01966-2. Tested in ELISA, Flow Cytometry, IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A01966-2

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

LIN-28

Synonyms

Protein lin-28 homolog A; Lin-28A; Zinc finger CCHC domain-containing protein 1; LIN28A; CSDD1; LIN28; ZCCHC1

Gene Name

LIN28A

Gene ID

79727

UniProt

Q9H9Z2

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human Lin28/LIN28A recombinant protein (Position: S3-N209) .

Clonality

Polyclonal

Tissue Specificity

Expressed in embryonic stem cells, placenta and testis. Tends to be up-regulated in HER2-overexpressing breast tumors.

Applications

WB, IHC, IF, Flow Cytometry, ELISA

Field of Research

Growth Factors/Hormones, Lipid Signaling, Signal Transduction, Signaling Pathway

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

RNA-binding protein that inhibits processing of pre-let-7 miRNAs and regulates translation of mRNAs that control developmental timing, pluripotency and metabolism. Seems to recognize a common structural G-quartet (G4) feature in its miRNA and mRNA targets (Probable) . 'Translational enhancer' that drives specific mRNAs to polysomes and increases the efficiency of protein synthesis. Its association with the translational machinery and target mRNAs results in an increased number of initiation events per molecule of mRNA and, inly, in mRNA stabilization. Binds IGF2 mRNA, MYOD1 mRNA, ARBP/36B4 ribosomal protein mRNA and its own mRNA. Essential for skeletal muscle differentiation program through the translational up-regulation of IGF2 expression. Suppressor of microRNA (miRNA) biogenesis, including that of let-7, miR107, miR-143 and miR-200c. Specifically binds the miRNA precursors (pre-miRNAs), recognizing an 5'-GGAG-3' motif found in pre-miRNA terminal loop, and recruits TUT4 AND tut7 uridylyltransferaseS. This results in the terminal uridylation of target pre-miRNAs. Uridylated pre-miRNAs fail to be processed by Dicer and undergo degradation. The repression of let-7 expression is required for normal development and contributes to maintain the pluripotent state by preventing let-7-mediated differentiation of embryonic stem cells. Localized to the periendoplasmic reticulum area, binds to a large number of spliced mRNAs and inhibits the translation of mRNAs destined for the ER, reducing the synthesis of transmembrane proteins, ER or Golgi lumen proteins, and secretory proteins. Binds to and enhances the translation of mRNAs for several metabolic enzymes, such as PFKP, PDHA1 or SDHA, increasing glycolysis and oxidative phosphorylation. Which, with the let-7 repression may enhance tissue repair in adult tissue.

References & Citations

1. Chang, H.-M., Triboulet, R., Thornton, J. E., Gregory, R. I. A role for the Perlman syndrome exonuclease Dis3l2 in the Lin28-let-7 pathway. Nature 497: 244-248, 2013. 2. Hanna, J., Saha, K., Pando, B., van Zon, J., Lengner, C. J., Creyghton, M. P., van Oudenaarden, A., Jaenisch, R. cell reprogramming is a stochastic process amenable to acceleration. Nature 462: 595-601, 2009. 3. Moss, E. G., Tang, L. Conservation of the heterochronic regulator Lin-28, its developmental expression and microRNA complementary sites. Dev. Biol. 258: 432-442, 2003. Note: Erratum: Dev. Biol. 262: 361 only, 2003.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

30570 MW

Observed Molecular Weight

28 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

Lin-28 homolog A

Subcellular Location

Nucleolus. Rough endoplasmic reticulum. Cytoplasm. P-body. Stress granule.

Isotype

Rabbit IgG

Contents

243