Anti-LRPPRC/GP130 Antibody Picoband®

About this product

Boster Bio Anti-LRPPRC/GP130 Antibody Picoband® catalog # A03264. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A03264

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Leucine-rich PPR motif-containing protein, mitochondrial is a protein that in humans is encoded by the LRPPRC gene. It is mapped to 2p21. This gene encodes a leucine-rich protein that has multiple pentatricopeptide repeats (PPR) . The precise role of this protein is unknown but studies suggest it may play a role in cytoskeletal organization, vesicular transport, or in transcriptional regulation of both nuclear and mitochondrial genes. The protein localizes primarily to mitochondria and is predicted to have an N-terminal mitochondrial targeting sequence. Mutations in this gene are associated with the French-Canadian type of Leigh syndrome.

Synonyms

Leucine-rich PPR motif-containing protein, mitochondrial; 130 kDa leucine-rich protein; LRP 130; GP130; LRPPRC; LRP130

Gene Name

LRPPRC

Gene ID

10128

UniProt

P42704

Host

Rabbit

Reactivity

Human, Mouse, Rat, Monkey

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human LRPPRC/GP130 recombinant protein (Position: A1281-S1394) .

Clonality

Polyclonal

Tissue Specificity

Expressed ubiquitously. Expression is highest in heart, skeletal muscle, kidney and liver, intermediate in brain, non-mucosal colon, spleen and placenta, and lowest in small intestine, thymus, lung and peripheral blood leukocytes.

Applications

ELISA, Flow Cytometry, IF, IHC, ICC, WB

Field of Research

Cancer, Cancer Metabolism, Energy Metabolism, Energy Transfer Pathways, Integration Of Energy, Integration Of Energy Metabolism, Metabolic Signaling Pathway, Metabolic Signaling Pathways, Metabolism, Mitochondrial, Mitochondrial Markers, Mitochondrial Metabolism, Pathways and Processes, Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

May play a role in RNA metabolism in both nuclei and mitochondria. In the nucleus binds to HNRPA1-associated poly (A) mRNAs and is part of nmRNP complexes at late stages of mRNA maturation which are possibly associated with nuclear mRNA export. May bind mature mRNA in the nucleus outer membrane. In mitochondria binds to poly (A) mRNA. Plays a role in translation or stability of mitochondrially encoded cytochrome c oxidase (COX) subunits. May be involved in transcription regulation. Cooperates with PPARGC1A to regulate certain mitochondrially encoded genes and gluconeogenic genes and may regulate docking of PPARGC1A to transcription factors. Seems to be involved in the transcription regulation of the multidrug-related genes MDR1 and MVP. Part of a nuclear factor that binds to the invMED1 element of MDR1 and MVP gene promoters. Binds single-stranded DNA.

References & Citations

1. Harmel, J., Ruzzenente, B., Terzioglu, M., Spahr, H., Falkenberg, M., Larsson, N.-G. The leucine-rich pentatricopeptide repeat-containing protein (LRPPRC) does not activate transcription in mammalian mitochondria. J. Biol. Chem. 288: 15510-15519, 2013. 2. Hou, J., Wang, F., McKeehan, W. L. Molecular cloning and expression of the gene for a major leucine-rich protein from human hepatoblastoma cells (HepG2) . In Vitro Cell Dev. Biol. Anim. 30A: 111-114, 1994. 3. Liu, L., McKeehan, W. L. Sequence analysis of LRPPRC and its SEC1 domain interaction partners suggest roles in cytoskeletal organization, vesicular trafficking, nucleocytosolic shuttling, and chromosome activity. Genomics 79: 124-136, 2002.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

158 kDa

Observed Molecular Weight

158 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

Leucine rich pentatricopeptide repeat containing

Subcellular Location

Mitochondrion. Nucleoplasm. Nucleus inner membrane. Nucleus outer membrane.

Isotype

Rabbit IgG

Contents

243