METTL14 Recombinant Rabbit Monoclonal Antibody [PSH07-85]

About this product

The METTL3-METTL14 heterodimer forms a N6-methyltransferase complex that methylates adenosine residues at the N (6) position of some mRNAs and regulates the circadian clock, differentiation of embryonic stem cells and cortical neurogenesis. In the heterodimer formed with METTL3, METTL14 constitutes the RNA-binding scaffold that recognizes the substrate rather than the catalytic core. N6-methyladenosine (m6A), which takes place at the 5'-[AG]GAC-3' consensus sites of some mRNAs, plays a role in mRNA stability and processing. M6A acts as a key regulator of mRNA stability by promoting mRNA destabilization and degradation (By similarity). In embryonic stem cells (ESCs), m6A methylation of mRNAs encoding key naive pluripotency-promoting transcripts results in transcript destabilization (By similarity). M6A regulates spermatogonial differentiation and meiosis and is essential for male fertility and spermatogenesis (By similarity). M6A also regulates cortical neurogenesis: m6A methylation of transcripts related to transcription factors, neural stem cells, the cell cycle and neuronal differentiation during brain development promotes their destabilization and decay, promoting differentiation of radial glial cells (By similarity).

Specifications

Catalog number

HA722910

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

KIAA1627 antibody MET14_HUMAN antibody Methyltransferase like 14 antibody Methyltransferase-like protein 14 antibody METTL14 antibody N6-adenosine-methyltransferase subunit METTL14 antibody OTTHUMP00000219837 antibody

Abbreviation

METTL14

Swiss Prot

Q9HCE5 Human, Q3UIK4 Mouse,

Cellular Locus

Nucleus.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat, Monkey

Immunogen

Recombinant protein within human METTL14 aa 1-150.

Isotype

IgG

Clone

PSH07-85

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IF-Cell, IHC-P, FC, IP

Positive Control

A431 cell lysate, Raji cell lysate, PC-12 cell lysate, COS-1 cell lysate, Mouse testis tissue lysate, Rat testis tissue lysate, A431, human kidney tissue, human lung tissue, mouse testis tissue, rat kidney tissue, rat lung tissue, rat testis tissue.

Concentration

1 mg/mL

Dilution

WB: 1:5,000 ;IF-Cell: 1:100 ;IHC-P: 1:200-1:1,000 ;FC: 1:1,000 ;IP: 1-2μg/sample

Purity

Protein A affinity purified.

Form

Liquid

Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 52 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

Yes