Anti-USP21 Antibody Picoband®

About this product

Boster Bio Anti-USP21 Antibody Picoband® catalog # A06639-1. Tested in ELISA, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A06639-1

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Ubiquitin carboxyl-terminal hydrolase 21 is a protein that in humans is encoded by the USP21 gene. It is mapped to 1q23.3. This gene encodes a member of the C19 peptidase family, also known as family 2 of ubiquitin carboxy-terminal hydrolases. The encoded protein cleaves ubiquitin from ubiquitinated proteins for recycling in intracellular protein degradation. The encoded protein is also able to release NEDD8, a ubiquitin-like protein, from NEDD8-conjugated proteins. This gene has been referred to as USP16 and USP23 but is now known as USP21. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Synonyms

Ubiquitin carboxyl-terminal hydrolase 21; Deubiquitinating enzyme 21; Ubiquitin thioesterase 21; Ubiquitin-specific-processing protease 21; USP21; USP23; PP1490

Gene Name

USP21

Gene ID

27005

UniProt

Q9UK80

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human USP21 recombinant protein (Position: A277-L565) .

Clonality

Polyclonal

Tissue Specificity

Highly expressed in heart, pancreas and skeletal muscle. Also expressed in brain, placenta, liver and kidney, and at very low level in lung.

Applications

ELISA, IF, ICC, WB

Field of Research

Cofactors, Vitamins/Minerals, Metabolism, Mitochondrial, Mitochondrial Markers, Mitochondrial Metabolism, Pathways and Processes, Signal Transduction, Vitamins/Minerals

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Deubiquitinates histone H2A, a specific tag for epigenetic transcriptional repression, thereby acting as a coactivator. Deubiquitination of histone H2A releaves the repression of di- and trimethylation of histone H3 at 'Lys-4', resulting in regulation of transcriptional initiation. Regulates gene expression via histone H2A deubiquitination. Also capable of removing NEDD8 from NEDD8 conjugates but has no effect on Sentrin-1 conjugates. Deubiquitinates BAZ2A/TIP5 leading to its stabilization.

References & Citations

1. Nakagawa, T., Kajitani, T., Togo, S., Masuko, N., Ohdan, H., Hishikawa, Y., Koji, T., Matsuyama, T., Ikura, T., Muramatsu, M., Ito, T. Deubiquitylation of histone H2A activates transcriptional initiation via trans-histone cross-talk with H3K4 di- and trimethylation. Genes Dev. 22: 37-49, 2008. 2. Smith, T. S., Southan, C. Sequencing, tissue distribution and chromosomal assignment of a novel ubiquitin-specific protease USP23. Biochim. Biophys. Acta 1490: 184-188, 2000. 3. Xu, G., Tan, X., Wang, H., Sun, W., Shi, Y., Burlingame, S., Gu, X., Cao, G., Zhang, T., Qin, J., Yang, J. Ubiquitin-specific peptidase 21 inhibits tumor necrosis factor alpha-induced nuclear factor kappa-B activation via binding to and deubiquitinating receptor-interacting protein 1. J. Biol. Chem. 285: 969-978, 2010.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

104553 MW

Observed Molecular Weight

63 kDa

Specificity

No cross reactivity with other proteins.

Gene Name Synonym

Ubiquitin specific peptidase 21

Subcellular Location

Nucleus. Cytoplasm.

Protein Name

AP-2 complex subunit beta

Isotype

Rabbit IgG

Contents

243