Anti-Vinculin/VCL Antibody Picoband®

About this product

Boster Bio Anti-Vinculin/VCL Antibody Picoband® catalog # A01207-1. Tested in ELISA, Flow Cytometry, IF, IHC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A01207-1

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

In mammalian cells, vinculin is a membrane-cytoskeletal protein in focal adhesion plaques that is involved in linkage of integrin adhesion molecules to the actin cytoskeleton. It is mapped to 10q22.2. Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Defects in VCL are the cause of cardiomyopathy dilated type 1W. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined.

Synonyms

Vinculin; Metavinculin; MV; VCL

Gene Name

VCL

Gene ID

7414

UniProt

P18206

Host

Rabbit

Reactivity

Human, Monkey, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human Vinculin/VCL recombinant protein (Position: M1-E149) .

Clonality

Polyclonal

Tissue Specificity

Metavinculin is muscle-specific.

Applications

WB, IHC, IF, Flow Cytometry, ELISA

Field of Research

Actin Binding Proteins, Actin, etc., Cardiovascular, Contractility, Cytoskeleton, Cytoskeleton/ECM, Microfilaments, Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Actin filament (F-actin) -binding protein involved in cell-matrix adhesion and cell-cell adhesion. Regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. May also play important roles in cell morphology and locomotion.

References & Citations

1. Bakolitsa, C., Cohen, D. M., Bankston, L. A., Bobkov, A. A., Cadwell, G. W., Jennings, L., Critchley, D. R., Craig, S. W., Liddington, R. C. Structural basis for vinculin activation at sites of cell adhesion. Nature 430: 583-586, 2004. 2. del Rio, A., Perez-Jimenez, R., Liu, R., Roca-Cusachs, P., Fernandez, J. M., Sheetz, M. P. Stretching single talin rod molecules activates vinculin binding. Science 323: 638-641, 2009. 3. Gimona, M., Small, J. V., Moeremans, M., Van Damme, J., Puype, M., Vandekerckhove, J. Porcine vinculin and metavinculin differ by a 68-residue insert located close to the carboxy-terminal part of the molecule. EMBO J. 7: 2329-2334, 1988.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

123799 MW

Observed Molecular Weight

124 kDa

Specificity

No cross reactivity with other proteins.

Applications Notes

6

Gene Name Synonym

Vinculin

Subcellular Location

Cell membrane. Peripheral membrane protein. Cytoplasmic side. Sarcolemma. Cytoskeleton. Adherens junction. Focal adhesion.

Protein Name

Vinculin

Isotype

Rabbit IgG

Contents

243