Anti-hnRNP D/AUF1/HNRNPD Antibody Picoband® (monoclonal, 2B12)

About this product

Boster Bio Anti-hnRNP D/AUF1/HNRNPD Antibody Picoband® (monoclonal, 2B12) catalog # M09982. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

M09982

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Heterogeneous nuclear ribonucleoprotein D0 (HNRNPD) also known as AU-rich element RNA-binding protein 1 (AUF1) is a protein that in humans is encoded by the HNRNPD gene. It is mapped to 4q21.22. This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs) . The hnRNPs are nucleic acid binding proteins and they complex with heterogeneous nuclear RNA (hnRNA) . These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has two repeats of quasi-RRM domains that bind to RNAs. It localizes to both the nucleus and the cytoplasm. This protein is implicated in the regulation of mRNA stability. Alternative splicing of this gene results in four transcript variants.

Synonyms

ATP synthase D chain mitochondrial antibody|ATP synthase H+ transporting mitochondrial F1F0 subunit antibody|ATP synthase H+ transporting mitochondrial F1F0 subunit d antibody|ATP synthase subunit d antibody|ATP synthase subunit d, mitochondrial antibody|ATP synthase, H+ transporting, mitochondrial F0 complex, subunit d antibody|ATP5H antibody|ATP5H_HUMAN antibody|ATP5JD antibody|ATPase subunit d antibody|ATPQ antibody|mitochondrial antibody|My032 protein antibody

Gene Name

HNRNPD

Gene ID

3184

UniProt

Q14103

Host

Mouse

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human hnRNP D/AUF1/HNRNPD recombinant protein (Position: E88-N246) .

Clonality

Monoclonal

Clone

Clone: 2B12

Tissue Specificity

Ubiquitously expressed with highest levels in spleen, thymus and immature brain.

Applications

WB, IHC, ICC, IF, Flow Cytometry

Field of Research

Chaperones, Protein Trafficking, Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Mitochondrial membrane ATP synthase (F (1) F (0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F (1) - containing the extramembraneous catalytic core, and F (0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F (1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F (0) domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha (3) beta (3) subcomplex and subunit a/ATP6 static relative to the rotary elements.

References & Citations

1. Arao Y, Kuriyama R, Kayama F, Kato S (August 2000) . A nuclear matrix-associated factor, SAF-B, interacts with specific isoforms of AUF1/hnRNP D. Arch. Biochem. Biophys. 380 (2) : 228–36. 2. Sinsimer KS, Gratacós FM, Knapinska AM, Lu J, Krause CD, Wierzbowski AV, Maher LR, Scrudato S, Rivera YM, Gupta S, Turrin DK, De La Cruz MP, Pestka S, Brewer G (September 2008) . Chaperone Hsp27, a novel subunit of AUF1 protein complexes, functions in AU-rich element-mediated mRNA decay. Mol. Cell. Biol. 28 (17) : 5223–37. 3. Panda AC, Abdelmohsen K, Yoon JH, Martindale JL, Yang X, Curtis J, Mercken EM, Chenette DM, Zhang Y, Schneider RJ, Becker KG, de Cabo R, Gorospe M (Aug 2014) . RNA-binding protein AUF1 promotes myogenesis by regulating MEF2C expression levels. Mol Cell Biol. 34 (16) : 3106–19.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Observed Molecular Weight

43-45 kDa

Applications Notes

6

Gene Name Synonym

ATP synthase, H+ transporting, mitochondrial Fo complex, subunit d

Subcellular Location

Mitochondrion. Mitochondrion inner membrane.

Protein Name

ATP synthase subunit d, mitochondrial

Isotype

Mouse IgG2a

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.