Anti-Glutaminase/GLS Antibody Picoband®

About this product

Boster Bio Anti-Glutaminase/GLS Antibody Picoband® catalog # A01272-2. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A01272-2

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

This gene encodes the K-type mitochondrial glutaminase. The encoded protein is an phosphate-activated amidohydrolase that catalyzes the hydrolysis of glutamine to glutamate and ammonia. This protein is primarily expressed in the brain and kidney plays an essential role in generating energy for metabolism, synthesizing the brain neurotransmitter glutamate and maintaining acid-base balance in the kidney. Alternate splicing results in multiple transcript variants.

Synonyms

[Pyruvate dehydrogenase (acetyl-transferring) ] kinase isozyme 1, mitochondrial; Pyruvate dehydrogenase kinase isoform 1; PDH kinase 1; PDK1; PDHK1

Gene Name

GLS

Gene ID

2744

UniProt

O94925

Host

Rabbit

Reactivity

Human, Mouse, Rat, Monkey

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human Glutaminase/GLS recombinant protein (Position: K396-N654) .

Clonality

Polyclonal

Tissue Specificity

Expressed predominantly in the heart. Detected at lower levels in liver, skeletal muscle and pancreas.

Applications

WB, IHC, ICC, IF, Flow Cytometry, ELISA

Field of Research

Metabolism, Metabolism Of Carbohydrates, Methylation, Mitochondrial, Mitochondrial Markers, Mitochondrial Metabolism, Oncoproteins/Suppressors, Pathways and Processes, Signal Transduction, Tumor Suppressors

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Kinase that plays a key role in regulation of glucose and fatty acid metabolism and homeostasis via phosphorylation of the pyruvate dehydrogenase subunits PDHA1 and PDHA2. This inhibits pyruvate dehydrogenase activity, and thereby regulates metabolite flux through the tricarboxylic acid cycle, down-regulates aerobic respiration and inhibits the formation of acetyl-coenzyme A from pyruvate. Plays an important role in cellular responses to hypoxia and is important for cell proliferation under hypoxia. Protects cells against apoptosis in response to hypoxia and oxidative stress.

References & Citations

1. Lynch, D. S., Chelban, V., Vandrovcova, J., Pittman, A., Wood, N. W., Houlden, H. GLS loss of function causes autosomal recessive spastic ataxia and optic atrophy. Ann. Clin. Transl. Neurol. 5: 216-221, 2018. 2. Modi, W. S., Pollock, D. D., Mock, B. A., Banner, C., Renauld, J.-C., Van Snick, J. Regional localization of the human glutaminase (GLS) and interleukin-9 (IL9) genes by in situ hybridization. Cytogenet. Cell Genet. 57: 114-116, 1991. 3. Rumping, L., Tessadori, F., Pouwels, P. J. W., Vringer, E., Wijnen, J. P., Bhogal, A. A., Savelberg, S. M. C., Duran, K. J., Bakkers, M. J. G., Ramos, R. J. J., Schellekens, P. A. W., Kroes, H. Y., and 16 others. GLS hyperactivity causes glutamate excess, infantile cataract and profound developmental delay. Hum. Molec. Genet. 28: 96-104, 2019.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

10044 MW

Observed Molecular Weight

65-73 kDa

Applications Notes

6

Gene Name Synonym

Pyruvate dehydrogenase kinase, isozyme 1

Subcellular Location

Mitochondrion matrix.

Protein Name

Acyl-CoA-binding protein

Isotype

Rabbit IgG

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.