Anti-GSDMD Antibody Picoband®

About this product

Boster Bio Anti-GSDMD Antibody Picoband® catalog # A02842. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A02842

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Gasdermin D is a member of the gasdermin family. Members of this family appear to play a role in regulation of epithelial proliferation. Gasdermin D has been suggested to act as a tumor suppressor. Alternatively spliced transcript variants have been described.

Synonyms

Gasdermin D, gasdermin-D

Gene Name

GSDMD

Gene ID

79792

UniProt

P57764

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human GSDMD recombinant protein (Position: M1-H484) .

Clonality

Polyclonal

Tissue Specificity

Brain, placental, and gut tissues.

Applications

ELISA, IF, IHC, ICC, WB, Flow Cytometry

Field of Research

Atherosclerosis, Cardiovascular, Fatty Acid Oxidation, Fatty Acids, Heart Disease, Lipid and Lipoprotein Metabolism, Lipids/Lipoproteins, Metabolic Signaling Pathways, Metabolism, Neurology Process, Neuroscience, Pathways and Processes, Redox Metabolism, Vascular Inflammation

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Receptor for MSH (alpha, beta and gamma) and ACTH. This receptor is mediated by G proteins which activate adenylate cyclase. Required for expression of anticipatory patterns of activity and wakefulness during periods of limited nutrient availability and for the normal regulation of circadian clock activity in the brain. .

References & Citations

1. Ruhl, S., Shkarina, K., Demarco, B., Heilig, R., Santos, J. C., Broz, P. ESCRT-dependent membrane repair negatively regulates pyroptosis downstream of GSDMD activation. Science 362: 956-960, 2018. 2. Shi, J., Zhao, Y., Wang, K., Shi, X., Wang, Y., Huang, H., Zhuang, Y., Cai, T., Wang, F., Shao, F. Cleavage of GSDMD by inflammatory caspases determines pyroptotic cell death. Nature 526: 660-665, 2015. 3. Zhu, Q., Zheng, M., Balakrishnan, A., Karki, R., Kanneganti, T.-D. Gasdermin D promotes AIM2 inflammasome activation and is required for host protection against Francisella novicida. J. Immun. 201: 3662-3668, 2018.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

36043 MW

Observed Molecular Weight

30, 53 kDa

Applications Notes

6

Gene Name Synonym

Melanocortin receptor 3

Subcellular Location

Cell membrane; Multi-pass membrane protein.

Protein Name

Melanocortin receptor 3

Isotype

Rabbit IgG

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.