Anti-non-muscle Myosin IIB/MYH10 Antibody Picoband®

About this product

Boster Bio Anti-non-muscle Myosin IIB/MYH10 Antibody Picoband® catalog # A02681-1. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A02681-1

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

This gene encodes a member of the myosin superfamily. The protein represents a conventional non-muscle myosin; it should not be confused with the unconventional myosin-10 (MYO10) . Myosins are actin-dependent motor proteins with diverse functions including regulation of cytokinesis, cell motility, and cell polarity. Mutations in this gene have been associated with May-Hegglin anomaly and developmental defects in brain and heart. Multiple transcript variants encoding different isoforms have been found for this gene.

Synonyms

T-cell surface glycoprotein CD3 epsilon chain; T-cell surface antigen T3/Leu-4 epsilon chain; CD3e; Cd3e

Gene Name

MYH10

Gene ID

4628

UniProt

P35580

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human non-muscle Myosin IIB/MYH10, identical to the related mouse and rat sequences.

Clonality

Polyclonal

Tissue Specificity

Highly expressed in epithelial tissues, particularly those of the gastrointestinal and respiratory tracts, such as large intestine and trachea, followed by kidney, small intestine, appendix and stomach.

Applications

WB, IHC, ICC, IF, Flow Cytometry

Field of Research

Adaptive Immunity, Hematopoietic Progenitors, Immunology, Stem Cells, T Cells

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

The CD3 complex mediates signal transduction, resulting in T-cell activation and proliferation. Required for normal immune responses.

References & Citations

1. Ma, X., Adelstein, R. S. A point mutation in Myh10 causes major defects in heart development and body wall closure. Circ. Cardiovasc. Genet. 7: 257-265, 2014. Note: Erratum: Circ. Cardiovasc. Genet. 7: 570 only, 2014. 2. Tuzovic, L., Yu, L., Zeng, W., Li, X., Lu, H., Lu, H.-M., Gonzalez, K., Chung, W. K. A human de novo mutation in MYH10 phenocopies the loss of function mutation in mice. Rare Dis. 1: e26144, 2013. Note: Electronic Article.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

33310 MW

Observed Molecular Weight

229 kDa

Applications Notes

6

Gene Name Synonym

CD3 antigen, epsilon polypeptide

Subcellular Location

Secreted. Cell membrane. Single-pass type I membrane protein. Apical cell membrane.

Isotype

Rabbit IgG

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.