- Catalog number
- A02476-1
- Size / count
- 100 µg/Vial
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- This gene encodes neutrophil cytosolic factor 2, the 67-kilodalton cytosolic subunit of the multi-protein NADPH oxidase complex found in neutrophils. This oxidase produces a burst of superoxide which is delivered to the lumen of the neutrophil phagosome. Mutations in this gene, as well as in other NADPH oxidase subunits, can result in chronic granulomatous disease, a disease that causes recurrent infections by catalase-positive organisms. Alternative splicing results in multiple transcript variants encoding different isoforms.
- Synonyms
- Syntenin-1; Melanoma differentiation-associated protein 9; MDA-9; Pro-TGF-alpha cytoplasmic domain-interacting protein 18; TACIP18; Scaffold protein Pbp1; Syndecan-binding protein 1; SDCBP; MDA9; SYCL
- Gene Name
- NCF2
- Gene ID
- 4688
- UniProt
- P19878
- Host
- Rabbit
- Reactivity
- Human, Mouse, Rat
- Cross Reactivity
- No cross-reactivity with other proteins.
- Immunogen
- E.coli-derived human NOXA2/p67phox/NCF2 recombinant protein (Position: M1-V526) .
- Clonality
- Polyclonal
- Tissue Specificity
- Expressed in lung cancers, including adenocarcinoma, squamous cell carcinoma and small-cell carcinoma. Widely expressed. Expressed in fetal kidney, liver, lung and brain. In adult highest expression in heart and placenta.
- Applications
- WB, IHC, Flow Cytometry, ELISA
- Field of Research
- Cell Type Marker, Neurogenesis, Neurology Process, Neuron Marker, Neuroscience, Synapse Marker
- Purification
- Immunogen affinity purified.
- Concentration
- Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
- Form
- Lyophilized
- Reconstitution
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
- Function
- Multifunctional adapter protein involved in diverse array of functions including trafficking of transmembrane proteins, neuro and immunomodulation, exosome biogenesis, and tumorigenesis. Positively regulates TGFB1-mediated SMAD2/3 activation and TGFB1-induced epithelial-to-mesenchymal transition (EMT) and cell migration in various cell types. May increase TGFB1 signaling by enhancing cell-surface expression of TGFR1 by preventing the interaction between TGFR1 and CAV1 and subsequent CAV1-dependent internalization and degradation of TGFR1. In concert with SDC1/4 and PDCD6IP, regulates exosome biogenesis. Regulates migration, growth, proliferation, and cell cycle progression in a variety of cancer types. In adherens junctions may function to couple syndecans to cytoskeletal proteins or signaling components. Seems to couple transcription factor SOX4 to the IL-5 receptor (IL5RA) . May also play a role in vesicular trafficking. Seems to be required for the targeting of TGFA to the cell surface in the early secretory pathway.
- References & Citations
- 1. Francke, U., Hsieh, C.-L., Foellmer, B. E., Lomax, K. J., Malech, H. L., Leto, T. L. Genes for two autosomal recessive forms of chronic granulomatous disease assigned to 1q25 (NCF2) and 7q11.23 (NCF1) . Am. J. Hum. Genet. 47: 483-492, 1990. 2. Noack, D., Rae, J., Cross, A. R., Munoz, J., Salmen, S., Mendoza, J. A., Rossi, N., Curnutte, J. T., Heyworth, P. G. Autosomal recessive chronic granulomatous disease caused by novel mutations in NCF-2, the gene encoding the p67-phox component of phagocyte NADPH oxidase. Hum. Genet. 105: 460-467, 1999. 3. Sancho-Shimizu, V., Malo, D. Sequencing, expression, and functional analyses support the candidacy of Ncf2 in susceptibility to Salmonella Typhimurium infection in wild-derived mice. J. Immun. 176: 6954-6961, 2006.
- Storage Conditions
- Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
- Calculated Molecular Weight
- 22105 MW
- Observed Molecular Weight
- 60 kDa
- Applications Notes
- 6
- Gene Name Synonym
- Syndecan binding protein
- Subcellular Location
- Nucleus. Exosome. Endoplasmic reticulum membrane. Peripheral membrane protein. Cytosol. Cytoskeleton. Cell membrane. Peripheral membrane protein.
- Isotype
- Rabbit IgG
- Contents
- Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
