Anti-P2RX7 Antibody Picoband®

About this product

Boster Bio Anti-P2RX7 Antibody Picoband® catalog # A01208-2. Tested in ELISA, WB applications. This antibody reacts with Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A01208-2

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

P2X purinoceptor 7, also called P2X7, is a protein that in humans is encoded by the P2RX7 gene. The product of this gene belongs to the family of purnoceptors for ATP. P2RX7 was mapped to chromosome 12q24 by fluorescence in situ hybridization. It is a receptor for ATP that acts as a ligand-gated ion channel. This gene is responsible for ATP-dependent lysis of macrophages through the formation of membrane pores permeable to large molecules. It could function in both fast synaptic transmission and the ATP-mediated lysis of antigen-presenting cells.

Synonyms

Vinculin; Metavinculin; MV; VCL

Gene Name

P2rx7

Gene ID

18439

UniProt

Q9Z1M0

Host

Rabbit

Reactivity

Mouse

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived mouse P2RX7 recombinant protein (Position: N380-E585) .

Clonality

Polyclonal

Tissue Specificity

Metavinculin is muscle-specific.

Applications

WB, ELISA

Field of Research

Actin Binding Proteins, Actin, etc., Cardiovascular, Contractility, Cytoskeleton, Cytoskeleton/ECM, Microfilaments, Signal Transduction

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Actin filament (F-actin) -binding protein involved in cell-matrix adhesion and cell-cell adhesion. Regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. May also play important roles in cell morphology and locomotion.

References & Citations

1. Adriouch, S., Dox, C., Welge, V., Seman, M., Koch-Nolte, F., Haag, F. Cutting edge: a natural P451L mutation in the cytoplasmic domain impairs the function of the mouse P2X7 receptor. J. Immun. 169: 4108-4112, 2002. 2. Cabrini, G., Falzoni, S., Forchap, S. L., Pellegatti, P., Balboni, A., Agostini, P., Cuneo, A., Castoldi, G., Baricordi, O. R., Di Virgilio, F. A his-155 to tyr polymorphism confers gain-of-function to the human P2X7 receptor of human leukemic lymphocytes. J. Immun. 175: 82-89, 2005. 3. Lemaire, I., Falzoni, S., Leduc, N., Zhang, B., Pellegatti, P., Adinolfi, E., Chiozzi, P., Di Virgilio, F. Involvement of the purinergic P2X7 receptor in the formation of multinucleated giant cells. J. Immun. 177: 7257-7265, 2006. 4. Weber, F. C., Esser, P. R., Muller, T., Ganesan, J., Pellegatti, P., Simon, M. M., Zeiser, R., Idzko, M., Jakob, T., Martin, S. F. Lack of the purinergic receptor P2X (7) results in resistance to contact hypersensitivity. J. Exp. Med. 207: 2609-2619, 2010.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

123799 MW

Observed Molecular Weight

68 kDa

Gene Name Synonym

Vinculin

Subcellular Location

Cell membrane. Peripheral membrane protein. Cytoplasmic side. Sarcolemma. Cytoskeleton. Adherens junction. Focal adhesion.

Protein Name

Vinculin

Isotype

Rabbit IgG

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.