Anti-P2Y14/P2ry14 Antibody Picoband®

About this product

Boster Bio Anti-P2Y14/P2ry14 Antibody Picoband® catalog # A09607-3. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A09607-3

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

The product of this gene belongs to the family of G-protein coupled receptors, which contains several receptor subtypes with different pharmacological selectivity for various adenosine and uridine nucleotides. This receptor is a P2Y purinergic receptor for UDP-glucose and other UDP-sugars coupled to G-proteins. It has been implicated in extending the known immune system functions of P2Y receptors by participating in the regulation of the stem cell compartment, and it may also play a role in neuroimmune function. Two transcript variants encoding the same protein have been identified for this gene.

Synonyms

Peflin; PEF protein with a long N-terminal hydrophobic domain; Penta-EF hand domain-containing protein 1; PEF1; ABP32; UNQ1845; PRO3573

Gene Name

P2ry14

Gene ID

140795

UniProt

Q9ESG6

Host

Rabbit

Reactivity

Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived mouse P2Y14/P2ry14 recombinant protein (Position: N17-L338) .

Clonality

Polyclonal

Tissue Specificity

Pre-B-cells and B-cells but not terminally differentiated plasma cells.

Applications

ELISA, Flow Cytometry, WB

Field of Research

Epigenetics and Nuclear Signaling, Mediator Complex, Transcription

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Calcium-binding protein that acts as an adapter that bridges unrelated proteins or stabilizes weak protein-protein complexes in response to calcium. Together with PDCD6, acts as calcium-dependent adapter for the BCR (KLHL12) complex, a complex involved in endoplasmic reticulum (ER) -Golgi transport by regulating the size of COPII coats. In response to cytosolic calcium increase, the heterodimer formed with PDCD6 interacts with, and bridges together the BCR (KLHL12) complex and SEC31 (SEC31A or SEC31B), promoting monoubiquitination of SEC31 and subsequent collagen export, which is required for neural crest specification. Its role in the heterodimer formed with PDCD6 is however unclear: some evidences show that PEF1 and PDCD6 work together and promote association between PDCD6 and SEC31 in presence of calcium. Other reports show that PEF1 dissociates from PDCD6 in presence of calcium, and may act as a negative regulator of PDCD6. Also acts as a negative regulator of ER-Golgi transport; possibly by inhibiting interaction between PDCD6 and SEC31.

References & Citations

1. Chambers, J. K., Macdonald, L. E., Sarau, H. M., Ames, R. S., Freeman, K., Foley, J. J., Zhu, Y., McLaughlin, M. M., Murdock, P., McMillan, L., Trill, J., Swift, A., and 14 others. A G protein-coupled receptor for UDP-glucose. J. Biol. Chem. 275: 10767-10771, 2000. 2. Charlton, M. E., Williams, A. S., Fogliano, M., Sweetnam, P. M., Duman, R. S. The isolation and characterization of a novel G protein-coupled receptor regulated by immunologic challenge. Brain Res. 764: 141-148, 1997. 3. Lee, B.-C., Cheng, T., Adams, G. B., Attar, E. C., Miura, N., Lee, S. B., Saito, Y., Olszak, I., Dombkowski, D., Olson, D. P., Hancock, J., Choi, P. S., Haber, D. A., Luster, A. D., Scadden, D. T. P2Y-like receptor, GPR105 (P2Y14), identifies and mediates chemotaxis of bone-marrow hematopoietic stem cells. Genes Dev. 17: 1592-1604, 2003.

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Observed Molecular Weight

40 kDa

Applications Notes

6

Gene Name Synonym

Penta-EF-hand domain containing 1

Subcellular Location

Endoplasmic reticulum. Cytoplasm. Membrane. Peripheral membrane protein. COPII-coated vesicle membrane. Peripheral membrane protein.

Isotype

Rabbit IgG

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.