- Catalog number
- A01415-1
- Size / count
- 100 µg/Vial
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- GATA2 (GATA binding protein 2) is a human gene which makes a protein called GATA binding protein 2 - a transcription factor. The GATA family of transcription factors, which contain zinc fingers in their DNA binding domain, have emerged as candidate regulators of gene expression in hematopoietic cells. Ciciotte et al. (1997) mapped the mouse Gata2 gene to chromosome 6 by study of DNA from an interspecific backcross panel. They pointed out that the human gene had been mapped to chromosome 3 by Dorfman et al. (1992) using DNA from a panel of 12 rodent/human hybrids containing various human chromosomes and applying Southern blot analysis. By exon trapping using a PAC contig spanning a breakpoint region associated with myeloid leukemia, Wieser et al. (2000) mapped the GATA2 gene to chromosome 3q21. GATA2 is transcribed from telomere to centromere. GATA2 is expressed in hematopoietic progenitors, including early erythroid cells, mast cells, and megakaryocytes, and also in nonhematopoietic embryonic stem cells.
- Synonyms
- Amiloride-sensitive sodium channel subunit alpha; Alpha-NaCH; Epithelial Na (+) channel subunit alpha; Alpha-ENaC; ENaCA; Nonvoltage-gated sodium channel 1 subunit alpha; SCNEA; SCNN1A; SCNN1
- Gene Name
- GATA2
- Gene ID
- 2624
- UniProt
- P23769
- Host
- Rabbit
- Reactivity
- Human
- Cross Reactivity
- No cross-reactivity with other proteins.
- Immunogen
- E.coli-derived human GATA2 recombinant protein (Position: D23-S288) .
- Clonality
- Polyclonal
- Tissue Specificity
- Expressed in the female reproductive tract, from the fimbrial end of the fallopian tube to the endometrium (at protein level) (PubMed:22207244) . Expressed in kidney (at protein level) . In the respiratory tract, expressed in the bronchial epithelium (at protein level) . Highly expressed in lung. Detected at intermediate levels in pancreas and liver, and at low levels in heart and placenta (PubMed:22207244) . in skin, expressed in keratinocytes, melanocytes and Merkel cells of the epidermal sub- layers, stratum basale, stratum spinosum and stratum granulosum (at protein level) (PubMed:28130590) . Expressed in the outer root sheath of the hair follicles (at protein level) (PubMed:28130590) . Detected in both peripheral and central cells of the sebaceous gland (at protein level) (PubMed:28130590) . Expressed by eccrine sweat glands (at protein level) (PubMed:28130590) . In skin, also expressed by arrector pili muscle cells and intradermal adipocytes (PubMed:28130590) . Isoform 1 and isoform 2 predominate in all tissues. Expression of isoform 3, isoform 4 and isoform 5 is very low or not detectable, except in lung and heart (PubMed:9575806) .
- Applications
- ELISA, Flow Cytometry, IF, ICC, WB
- Field of Research
- Cell Type Markers, Hematopoietic Progenitors, Immunology, Lymphoid, Myeloid, NK Cells, Stem Cells
- Purification
- Immunogen affinity purified.
- Concentration
- Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
- Form
- Lyophilized
- Reconstitution
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
- Function
- Sodium permeable non-voltage-sensitive ion channel inhibited by the diuretic amiloride. Mediates the electrodiffusion of the luminal sodium (and water, which follows osmotically) through the apical membrane of epithelial cells. Plays an essential role in electrolyte and blood pressure homeostasis, but also in airway surface liquid homeostasis, which is important for proper clearance of mucus. Controls the reabsorption of sodium in kidney, colon, lung and eccrine sweat glands. Also plays a role in taste perception.
- References & Citations
- 1. Ciciotte, S. L., Tsai, F.-Y., Peters, L. L.Gata2 maps to mouse chromosome 6.Mammalian Genome 8: 69-70, 1997. 2. Dorfman, D. M., Wilson, D. B., Bruns, G. A., Orkin, S. H.Human transcription factor GATA-2: evidence for regulation of preproendothelin-1 gene expression in endothelial cells.J. Biol. Chem. 267: 1279-1285, 1992. 3. Wieser, R., Volz, A., Vinatzer, U., Gardiner, K., Jager, U., Mitterbauer, M., Ziegler, A., Fonatsch, C.Transcription factor GATA-2 gene is located near 3q21 breakpoints in myeloid leukemia.Biochem. Biophys. Res. Commun. 273: 239-245, 2000.
- Storage Conditions
- Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
- Observed Molecular Weight
- 51 kDa
- Applications Notes
- 6
- Gene Name Synonym
- Sodium channel epithelial 1 alpha subunit
- Subcellular Location
- Apical cell membrane.
- Isotype
- Rabbit IgG
- Contents
- Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
